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Effects of different Sertoli cell types on the maintenance of adult spermatogonial stem cells in vitro

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Abstract

Spermatongonial stem cells (SSCs) are unique testis cells that are able to proliferate, differentiate, and transmit genetic information to the next generation. However, the effect of different Sertoli cell types on the expression of specific SSC genes is not yet well understood. In this study, we compare the in vitro effect of adult Sertoli cells, embryonic Sertoli cells, and TM4 (a Sertoli cell line) as feeder layers on the expression of SSC genes. SSCs were isolated from the testis of adult male mice and purified by differential plating. Following enrichment, SSCs were cultivated for 1 and 2 wk in the presence of various feeders. The expression of SSC-specific genes (Mvh, ZBTB, and c-kit) was evaluated by real-time polymerase chain reaction. Our results revealed that expression of the specific SSC genes was significantly higher in the embryonic Sertoli cells after 1 and 2 wk compared to the adult Sertoli cells and the TM4 group. Our finding suggest that co-culturing of SSCs with embryonic Sertoli cells is helpful for in vitro cultivation of SSCs and might improve the self-renewal of these stem cells.

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Acknowledgements

This study was financially supported by the research deputy of Arak University of Medical Sciences and Health Services Agent No. 805.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Maryam Baazm, Saeid Babaie & Parvindokht Bayat

  2. Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Farideh Jalali Mashayekhi

  3. Institute of Neuroanatomy, RWTH Aachen University, 52074, Aachen, Germany

    Cordian Beyer & Adib Zendedel

  4. Department of Anatomy, School of Medicine, Giulan University of Medical Sciences, Rasht, Iran

    Adib Zendedel

Authors
  1. Maryam Baazm
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  2. Farideh Jalali Mashayekhi
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  3. Saeid Babaie
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  4. Parvindokht Bayat
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  5. Cordian Beyer
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  6. Adib Zendedel
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Correspondence to Adib Zendedel.

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Editor: Tetsuji Okamoto

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Baazm, M., Mashayekhi, F.J., Babaie, S. et al. Effects of different Sertoli cell types on the maintenance of adult spermatogonial stem cells in vitro. In Vitro Cell.Dev.Biol.-Animal 53, 752–758 (2017). https://doi.org/10.1007/s11626-017-0172-z

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  • DOI: https://doi.org/10.1007/s11626-017-0172-z

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