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Simultaneous application of cisplatin and static magnetic field enhances oxidative stress in HeLa cell line

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Abstract

In this study, we reported the effects of simultaneous application of static magnetic field (SMF) and cisplatin as an anticancer drug on the oxidative stress in human cervical cancer (HeLa) cell line and normal skin fibroblast cells (Hu02). The cells were exposed to different SMF intensities (7, 10, and 15 mT) for 24 and 48 h. IC50 concentrations of cisplatin were obtained by MTT assay. The cytotoxic effects of combined treatment were studied by measuring the intracellular reactive oxygen species content using flow cytometric method and estimation of membrane lipid peroxidation by spectrophotometry. Statistical analysis was assessed using one-way repeated measures analysis of variance (ANOVA) followed by Tukey’s test. Based on the obtained results, the highest and lowest death rate, respectively, in HeLa and Hu02 cell lines was observed at the intensity of 10 mT. Also, we found that membrane lipid peroxidation in cancer cells is higher than that of normal counterparts. SMF potently sensitized human cervical cancer cells to cisplatin through reactive oxygen species (ROS) accumulation while it had small effects on normal cells. The combination of both treatments for 48 h led to a marked decrease in the viability percentage of HeLa cells by about 89% compared to untreated cells. This study suggests that conjugation of both physical and chemical treatments could increase the oxidative stress in HeLa cell line and among three optional intensities of SMF, the intensity of 10 mT led to the higher damage to cancer cells in lower doses of drug.

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Acknowledgment

The authors gratefully acknowledge the Research Council of Arak University of Medical Sciences (Grant Number: 1095) for the financial support and Dr. Nazanin Haghighat for her essential advice and comments. This work was performed in partial fulfillment of the requirements for MSc of Samaneh Kamalipooya, in School of Medicine, Arak University of Medical Sciences, Arak, Iran.

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Authors and Affiliations

  1. Department of Biotechnology, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Samaneh Kamalipooya

  2. Faculty of Bioscience, Department of Biophysics, Tarbiat Modares University, Tehran, Iran

    Parviz Abdolmaleki & Zahra Salemi

  3. Department of Biochemistry, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Fatemeh Javani Jouni

  4. Faculty of Pharmacology, Department of Pharmacology and Toxicology, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran

    Jaber Zafari

  5. Department of Medical physics and physiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran

    Homa Soleimani

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  1. Samaneh Kamalipooya
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  2. Parviz Abdolmaleki
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  3. Zahra Salemi
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  4. Fatemeh Javani Jouni
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  5. Jaber Zafari
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  6. Homa Soleimani
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Correspondence to Homa Soleimani.

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Editor: Tetsuji Okamoto

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Kamalipooya, S., Abdolmaleki, P., Salemi, Z. et al. Simultaneous application of cisplatin and static magnetic field enhances oxidative stress in HeLa cell line. In Vitro Cell.Dev.Biol.-Animal 53, 783–790 (2017). https://doi.org/10.1007/s11626-017-0148-z

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