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Lipoprotein profiles of hepatic cell lines at various stages of differentiation

  • Akira Sasaki
  • Fumiko Kimura
  • Mizuho Miura
  • Gen Toshima
  • Junichiro Takahashi
  • Sayuri Maruya
  • Masayuki Kobayashi
  • Keishi Hata
Letter to the Editor

Abstract

We studied the lipoprotein profiles of human hepatic cells at various stages of differentiation. The production of three major classes of lipoproteins, very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), was detected in three well-differentiated human hepatoma cell lines and primary human hepatocytes; however, these lipoproteins were not detected in the culture medium in which undifferentiated hepatoma cell lines were grown. Reverse transcription polymerase chain reaction analysis demonstrated that the expression levels of apolipoprotein A1 (ApoA1), ApoB100, and microsomal triglyceride transfer protein (MTP) were markedly lower in the undifferentiated hepatoma cell lines than in the well-differentiated hepatoma cell lines and primary hepatocytes. These results indicate that apolipoprotein synthesis, and triglyceride-transport by MTP might be rate-limiting steps in lipoprotein production in mature hepatic cells.

Keywords

Lipoprotein Hepatic cell differentiation Apolipoprotein 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11626_2016_91_MOESM1_ESM.docx (25 kb)
Table S1 (DOCX 25 kb)
11626_2016_91_MOESM2_ESM.pptx (68 kb)
Figure S1 (PPTX 67 kb)

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Copyright information

© The Society for In Vitro Biology 2016

Authors and Affiliations

  • Akira Sasaki
    • 1
    • 4
  • Fumiko Kimura
    • 2
  • Mizuho Miura
    • 2
  • Gen Toshima
    • 2
  • Junichiro Takahashi
    • 2
  • Sayuri Maruya
    • 3
  • Masayuki Kobayashi
    • 4
  • Keishi Hata
    • 1
  1. 1.Akita Research Institute of Food and BrewingAkitaJapan
  2. 2.Skylight BiotechAkitaJapan
  3. 3.ORIENTAL YEAST Co., Ltd.TokyoJapan
  4. 4.Graduate School of Bioresource ScienceAkita Prefectural UniversityAkitaJapan

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