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Potential efficacy of Lactobacillus casei IBRC_M10711 on expression and activity of insulin degrading enzyme but not insulin degradation

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Abstract

Type 2 diabetes (T2D) is a condition with insufficient insulin production or in the setting of insulin resistance with many origins including intestinal microbiota-related molecular mechanism. Insulin-degrading enzyme (IDE) is responsible for insulin breakdown in various tissues and is known as a potential drug target for T2D. Here, we assessed the effects of cell-free supernatant (CFS) and UV-killed Lactobacillus casei IBRC_M10711 on IDE expression, IDE activity, and insulin degradation in Caco-2 cell line. It was found that CFS and UV-killed L. casei IBRC_M10711 led to lower expression of IDE. UV-killed L. casei IBRC_M10711 significantly inhibited IDE activity but CFS did not. Insulin degradation was affected with none of them. In conclusion, L. casei IBRC_M10711 is effective on IDE expression and its activity, but not on insulin degradation. Future studies are recommended to explore the effect of this probiotic on other substrates of IDE.

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Acknowledgments

This research study is funded by the Tehran University of Medical Sciences, International Campus (TUMS-IC). The authors thank the staffs of Pharmacology and Toxicology laboratory, Faculty of Pharmacy, for their collaborations.

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Authors and Affiliations

  1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran

    Nadia Neyazi, Taiebeh Mohammadi Farsani, Zahra Nouri & Mohammad Reza Khorramizadeh

  2. Department of Oncology, Kabul Medical University, Jamal Mena, Kabul, Afghanistan

    Nadia Neyazi

  3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Hossein Ghahremani

  4. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Hossein Ghahremani & Elahe Motevaseli

  5. Biosensor Research Center, Endocrinilogy and Metabolism Molecular-Cellular Sciences Institute, EMRI, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Khorramizadeh

  6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Roksana Tajerian

  7. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Elahe Motevaseli

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  1. Nadia Neyazi
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  2. Taiebeh Mohammadi Farsani
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  4. Mohammad Hossein Ghahremani
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Correspondence to Elahe Motevaseli.

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Editor: Tetsuji Okamoto

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Neyazi, N., Mohammadi Farsani, T., Nouri, Z. et al. Potential efficacy of Lactobacillus casei IBRC_M10711 on expression and activity of insulin degrading enzyme but not insulin degradation. In Vitro Cell.Dev.Biol.-Animal 53, 12–19 (2017). https://doi.org/10.1007/s11626-016-0083-4

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