Abstract
In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
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This study received 4th Mandom International Research Grants on Alternative to Animal Experiments and a public welfare labor science research expenses subsidy (H19-medicine public-003 from 2007 to 2009, and H22-medicine public-002 from 2010 to 2012).
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Editor: Tetsuji Okamoto
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Yamamoto, N., Kato, Y., Sato, A. et al. Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. In Vitro Cell.Dev.Biol.-Animal 52, 742–748 (2016). https://doi.org/10.1007/s11626-016-0038-9
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DOI: https://doi.org/10.1007/s11626-016-0038-9