Abstract
Total Panax notoginseng saponin (PNS) has been extensively used to treat a variety of diseases, such as bone fractures, soft tissue injuries, etc. In this study, mouse calvaria-original osteoblastic MC3T3-E1 cells were cultured in various concentrations of PNS (0.005–5 mg/mL) during the period (1, 5, 14, and 23 d). At the endpoint, the osteogenic capacity of MC3T3-E1 cells was investigated by measuring the alkaline phosphatase (ALP) activity, the deposited calcium, and the expression of osteogenic-related markers, including bone collagen type 1 (Col1) and osteocalcin (OCN). Compared with all groups in each period, the most pronounced effect was observed at the concentration range between 0.05 and 0.5 mg/mL (P < 0.05) and the cell proliferation with PNS treatment was found during the whole osteogenic period. Moreover, cellular ALP activity with PNS was increased during 7, 14, and 21 d and cell mineralization with PNS was enhanced in 14 and 21 d. Furthermore, the differentiation markers Col1 and OCN increased in the PNS-treated cells. Our work suggests that PNS may stimulate the osteogenesis process which contains osteoblastic proliferation, differentiation, and mineralization by increasing cellular ALP activity, extracellular matrix mineralization, and osteoblast-associated molecules in the osteoblasts.
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Acknowledgments
This study was supported by the Natural Science Foundation of China (No. 81001225), the international Co-operative Fund in Xian Jiaotong University (No. 08143004), the Fundamental Research Funds for the Central Universities (No. 08140003), and Integrative Medicine Research and Innovation Team of Degenerative Bone Disease Prevention, Shaanxi Traditional Chinese Medicine College (2013KCT-26).
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Editor: T. Okamoto
Zhe Ji and Yizhao Cheng contributed equally to this work.
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Ji, Z., Cheng, Y., Yuan, P. et al. Panax notoginseng stimulates alkaline phosphatase activity, collagen synthesis, and mineralization in osteoblastic MC3T3-E1 cells. In Vitro Cell.Dev.Biol.-Animal 51, 950–957 (2015). https://doi.org/10.1007/s11626-015-9915-x
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DOI: https://doi.org/10.1007/s11626-015-9915-x