Abstract
Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the lymphoid organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the lymphoid organ of L. vannamei.
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References
Castille FL, Lawrence AL (1989) Relationship between maturation and biochemical composition of the gonads and digestive glands of the shrimps Penaeus aztecus ives and Penaeus setiferus. J Crustac Biol 9:202–211
Chen SN, Chi SC, Kou GH, Liao IC (1986) Cell culture from tissues of grass shrimp, Penaeus monodon. Fish Pathol 21:161–166
Chen SN, Kou GH (1989) Infection of cultured cells from the lymphoid organ of Penaeus monodon Fabricius by monodon-type baculovirus (MBV). J Fish Dis 12:73–76
Escamilla-Chimal EG, Van Herp F, Fanjul-Moles ML (2001) Daily variations in crustacean hyperglycaemic hormone and serotonin immunoreactivity during the development of crayfish. J Exp Biol 204(6):1073–1081
Fisher LR, Kon SK, Thompson SY (1952) Vitamin A and carotenoids in certain invertebrates I. Marine Crustacea. J Mar Biol Assoc U K 31(02):229–258
George SK, Dhar AK (2010) An improved method of cell culture system from eye stalk, hepatopancreas, muscle, ovary, and hemocytes of Penaeus vannamei. In Vitro Cell Dev Biol Anim 46:801–810
Goldsmith TH, Cronin TW (1993) The retinoids of seven species of mantis shrimp. Vis Neurosci 10(05):915–920
Grace TDC (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature 195:788–789
Gstraunthaler G (2003) Alternatives to the use of fetal bovine serum: serum-free cell culture. Altex 20(4):275–281
Han Q, Li P, Lu X, Guo Z, Guo H (2013) Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis. Aquaculture 410:101–113
Itami T, Maeda M, Kondo M, Takahashi Y (1999) Primary culture of lymphoid organ cells and haemocytes of kuruma shrimp, Penaeus japonicus. Methods Cell Sci 21:237–244
Jose S, Jayesh P, Sudheer NS, Poulose G, Mohandas A, Philip R, Bright Singh IS (2012) Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression. J Fish Dis 35:321–334
Kamemoto FI, Oyama SN (1985) Neuroendocrine influence on effector tissues of hydromineral balance in crustaceans. Curr Trends Comp Endocrinol: 883–886
Kasornchandra J, Boonyaratpalin S (1998) Primary shrimp cell culture: applications for studying white spot syndrome virus (WSSV). Adv Shrimp Biotechnol: 273–276
LeBlanc GA (2007) Crustacean endocrine toxicology: a review. Ecotoxicology 16(1):61–81
Li W, Nguyen VT, Corteel M, Dantas-Lima JJ, Thuong KV, Tuan VV, Bossier P, Sorgeloos P, Nauwynck H (2014) Characterization of a primary cell culture from lymphoid organ of Litopenaeus vannamei and use for studies on WSSV replication. Aquaculture 433:157–163
Lightner DV, Redman RM (1998) Shrimp diseases and current diagnostic methods. Aquaculture 164(1):201–220
Nadala EC, Lu Y, Loh PC (1993) Primary culture of lymphoid, nerve, and ovary cells from Penaeus stylirostris and Penaeus vannamei. In Vitro Cell Dev Biol Anim 29:620–622.
Rankin SM, Bradfield JY, Keeley LL (1989) Ovarian protein synthesis in the South American white shrimp, Penaeus vannamei, during the reproductive cycle. Invertebr Reprod Dev 15(1):27–33
Ravid T, Tietz A, Khayat M, Boehm E, Michelis R, Lubzens E (1999) Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus (De Haan). J Exp Biol 202(13):1819–1829
Shiau SY, Chen Y (2000) Estimation of the dietary vitamin A requirement of juvenile grass shrimp, Penaeus monodon. J Nutr 130(1):90–94
Tapay LM, Lu Y, Brock JA, Nadala ECB, Loh PC (1995) Transformation of primary cultures of shrimp (Penaeus stylirostris) lymphoid (Oka) organ with Simian virus-40 (T) antigen. Soc Exp Biol Med 209:73–78
Teshima SI, Kanazawa A (1983) Variation in lipid composition during the ovarian maturation of the shrimp. Bull Jpn Soc Sci Fish 49:957–962
Tong S, Miao HZ (1996) Attempts to initiate cell cultures from Penaeus chinensis tissues. Aquaculture 147(3):151–157
Wanlem S, Supamattaya K, Tantikitti C, Prasertsan P, Graidist P (2011) Expression and applications of recombinant crustacean hyperglycemic hormone from eyestalks of white shrimp (Litopenaeus vannamei) against bacterial infection. Fish Shellfish Immunol 30(3):877–885
Acknowledgments
The first author was supported by a scholarship from the Chinese Scholarship Council (CSC, file no. 2010633043). The authors thankfully acknowledge M. Wille and J. Desmyter for helping with shrimp cultures. We thank Xiaoyun Yang for her suggestions on this paper.
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Li, W., Van Tuan, V., Van Thuong, K. et al. Eye extract improves cell migration out of lymphoid organ explants of L. vannamei and viability of the primary cell cultures. In Vitro Cell.Dev.Biol.-Animal 51, 651–654 (2015). https://doi.org/10.1007/s11626-015-9882-2
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DOI: https://doi.org/10.1007/s11626-015-9882-2