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A new continuous cell line from Blaps rhynchoptera Fairmaire (Coleoptera: Tenebrionidae)

  • Xin Zhang
  • Ying FengEmail author
  • Wei-Feng Ding
  • Xian Li
  • Cheng-Ye Wang
Article

Abstract

The first continuous cell line from the neonate larval tissues of Blaps rhynchoptera, which has been used as a folk medicine in Yunnan Province, China, was established and designated RIRI-BR1. This cell line was serially subcultured in Schneider’s medium supplemented with 15% fetal bovine serum (FBS). The cells grew adherent to culture flasks and exhibited spindle-like and polygonal shapes. The growth rate was determined at the 50th passage, and the population doubling time was calculated to be 79.5 h. The post-thaw viability of the cell line at different passages showed that the cells from higher passages could be recovered easier after cryopreservation than the cells from lower passages. The average chromosome numbers from cells of the RIRI-BR1 cell line at passages 5 to 50 ranged from 12 to 130. The rDNA internal transcribed spacer (ITS) sequence analysis indicated that the RIRI-BR1 cell line was derived from B. rhynchoptera.

Keywords

Blaps rhynchoptera Coleoptera Cell line Characterization 

Notes

Acknowledgments

This work was supported by grants from the National Ministry of Forestry of China (No. 2013-4-53) and the Chinese Academy of Forestry (No. riricaf2012002M).

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Copyright information

© The Society for In Vitro Biology 2014

Authors and Affiliations

  • Xin Zhang
    • 1
  • Ying Feng
    • 1
    Email author
  • Wei-Feng Ding
    • 1
  • Xian Li
    • 1
  • Cheng-Ye Wang
    • 1
  1. 1.Research Institute of Resource InsectsChinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry AdministrationKunmingChina

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