Abstract
We have previously demonstrated that nestin-expressing multipotent hair follicle stem cells are located above the hair follicle bulge and can differentiate into neurons and other cell types in vitro. The nestin-expressing hair follicle stem cells promoted the recovery of pre-existing axons when they were transplanted to the severed sciatic nerve or injured spinal cord. We have also previously demonstrated that the whisker hair follicle contains nestin-expressing stem cells in the dermal papilla (DP) as well as in the bulge area (BA), but that their origin is in the BA. In the present study, we established the technique of long-term Gelfoam® histoculture of whiskers isolated from transgenic mice in which nestin drives green fluorescent protein (ND-GFP). Confocal imaging was used to monitor ND-GFP-expressing stem cells trafficking in real time between the BA and DP to determine the fate of the stem cells. It was observed over a 2-week period that the stem cells trafficked from the BA toward the DP area and extensively grew out onto Gelfoam® forming nerve-like structures. This new method of long-term histoculture of whiskers from ND-GFP mice will enable the extensive study of the behavior of nestin-expressing multipotent stem cells of the hair follicle.
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Amoh Y.; Kanoh M.; Niiyama S.; Kawahara K.; Satoh Y.; Katsuoka K.; Hoffman R. M. Human and mouse hair follicles contain both multipotent and monopotent stem cells. Cell Cycle 8: 176–177; 2009.
Amoh Y.; Li L.; Campillo R.; Kawahara K.; Katsuoka K.; Penman S.; Hoffman R. M. Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves. Proc. Natl. Acad. Sci. USA 102: 17734–17738; 2005a.
Amoh Y.; Li L.; Katsuoka K.; Hoffman R. M. Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function. Cell Cycle 7: 1865–1869; 2008.
Amoh Y.; Li L.; Katsuoka K.; Penman S.; Hoffman R. M. Multipotent nestin-positive, keratin-negative hair-follicle-bulge stem cells can form neurons. Proc. Natl. Acad. Sci. USA 102: 5530–5534; 2005b.
Biernaski J.; Paris M.; Morozova O.; Fagan B. M.; Marra M.; Pevny L.; Miller F. D. SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell 5: 610–623; 2009.
Hoffman R. M. Histocultures and their use. In: Encyclopedia of life sciences. John Wiley and Sons; Ltd.: Chichester; 2010; published online (cover story). doi:10.1002/9780470015902.a0002573.pub2
Li L.; Mignone J.; Yang M.; Matic M.; Penman S.; Enikolopov G.; Hoffman R. M. Nestin expression in hair follicle sheath progenitor cells. Proc. Natl. Acad. Sci. USA 100: 9958–9961; 2003.
Liu F.; Uchugonova A.; Kimura H.; Zhang C.; Zhao M.; Zhang L. et al. The bulge area is the major hair follicle source of nestin-expressing pluripotent stem cells which can repair the spinal cord compared to the dermal papilla. Cell Cycle 10: 830–839; 2011.
Philpott M. P.; Green M. R.; Kealey T. Human hair growth in vitro. J. Cell Sci. 97(pt 3): 463–471; 1990.
Yu H.; Fang D.; Kumar S. M.; Li L.; Nguyen T. K.; Acs G. et al. Isolation of a novel population of multipotent adult stem cells from human hair follicles. Am. J. Path. 168: 1879–1888; 2006.
Yu H.; Kumar S. M.; Kossenkov A. V.; Showe L.; Xu X. W. Stem cells with neural crest characteristics derived from the bulge region of cultured human hair follicles. J. Investig. Derm. 130: 1227–1236; 2010.
Uchugonova A.; Duong J.; Zhang N.; König K.; Hoffman R. M. The bulge area is the origin of nestin-expressing pluripotent stem cells of the hair follicle. J. Cell. Biochem. 112: 2046–2050; 2011.
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Editor: J. Denry Sato
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Duong, J., Mii, S., Uchugonova, A. et al. Real-time confocal imaging of trafficking of nestin-expressing multipotent stem cells in mouse whiskers in long-term 3-D histoculture. In Vitro Cell.Dev.Biol.-Animal 48, 301–305 (2012). https://doi.org/10.1007/s11626-012-9514-z
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DOI: https://doi.org/10.1007/s11626-012-9514-z