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Comparing the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells and bone marrow mononuclear cells to iPSCs

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Abstract

Induced pluripotent stem cells have been derived from various cell types via the ectopic expression of a cocktail of transcription factors. Previous studies have reported that induced pluripotent stem cells can be differentiated into multiple somatic cells, providing an invaluable resource in regenerative medicine. In this study, we compared the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells, and mouse bone marrow mononuclear cells by counting the number of alkaline phosphatase staining positive clones on day 15 after induced pluripotent stem cells induction. We found that a very low number of alkaline phosphatase-staining positive clones were derived from mouse bone marrow mesenchymal stem cells. We then evaluated the pluripotency of the clones by detecting the expression of embryonic stem cells markers and assessing their ability to form embryoid bodies and teratomas. Mouse bone marrow mesenchymal stem cells population is more homogeneous than mouse bone marrow mononuclear cells, which includes a variety of cell types. Our study indicated that the extremely low efficiency of mouse bone marrow mesenchymal stem cells induction implies that mouse bone marrow mesenchymal stem cells may not be a suitable cell type for the induction of induced pluripotent stem cells unless the efficiency of induction can be improved.

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Acknowledgments

This research was supported by grants from National Natural Science Foundation of China (no. 81070654/H0713), the Science and Technology Innovation Project of Jiangsu Province for Postgraduates (no. CXZZ11_0643) and the Science and Technology Innovation Project of Nantong University for Postgraduates (no. YKC11035). We acknowledge the technical assistance from Laboratory of Gynecology and Obstetrics (Affiliated Hospital of Nantong University), School of Life Sciences (Nantong University), SiDanSai Stem Cell Technology CO., LTD and Key Laboratory of Neuroregeneration (Nantong University).

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Authors and Affiliations

  1. Department of General Surgery, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, People’s Republic of China

    Lei Wang, Mingyan Zhu, Qingsong Guo, Xiangjun Fan, Shajun Zhu, Yao Wang, Yan Huang & Zhiwei Wang

  2. Department of surgical comprehensive laboratory, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, People’s Republic of China

    Yuhua Lu

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  1. Lei Wang
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  2. Mingyan Zhu
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  3. Qingsong Guo
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  4. Xiangjun Fan
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  5. Yuhua Lu
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  6. Shajun Zhu
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  7. Yao Wang
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  8. Yan Huang
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  9. Zhiwei Wang
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Corresponding author

Correspondence to Zhiwei Wang.

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Editor: T. Okamoto

Lei Wang and Mingyan Zhu contributed equally to this work.

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Wang, L., Zhu, M., Guo, Q. et al. Comparing the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells and bone marrow mononuclear cells to iPSCs. In Vitro Cell.Dev.Biol.-Animal 48, 236–243 (2012). https://doi.org/10.1007/s11626-012-9493-0

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  • DOI: https://doi.org/10.1007/s11626-012-9493-0

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