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Establishment of a Chinook salmon cell line with an inducible gene expression system

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Abstract

We have isolated a stable recombinant cell line CHSE-TOF5 derived from the Chinook salmon (Oncorhynchus tshawytscha) embryo cells for use as an inducible expression system. The cells were transfected with the pTet-Off plasmid from the Tet On/Off Clontech system, carrying a G418 resistance gene. Several G418-resistant clones were subcultured and characterised by quantitative PCR (qPCR) and by transient transfection. The level of expression of transcriptional activator was measured by qPCR in a number of isolated clones, and transient transfection with a pTRE2-hyg-LUC plasmid was used to evaluate the inducibility of these clones. A clone was selected for its relative fast cell growth and good level of inducibility. This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of proteins from fish or fish pathogens.

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Authors and Affiliations

  1. Immunology and Infection, Marine Scotland, Marine Laboratory, PO Box 101, 375 Victoria Road, Aberdeen, AB11 9DB, UK

    Bertrand Collet & Katherine Lester

Authors
  1. Bertrand Collet
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  2. Katherine Lester
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Correspondence to Bertrand Collet.

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Editor: T. Okamoto

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Collet, B., Lester, K. Establishment of a Chinook salmon cell line with an inducible gene expression system. In Vitro Cell.Dev.Biol.-Animal 47, 695–697 (2011). https://doi.org/10.1007/s11626-011-9466-8

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  • DOI: https://doi.org/10.1007/s11626-011-9466-8

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