Long-term cultivation of in vitro Apis mellifera cells by gene transfer of human c-myc proto-oncogene

  • Yasuko Kitagishi
  • Naoko Okumura
  • Hitomi Yoshida
  • Yuri Nishimura
  • Jun-ichi Takahashi
  • Satoru Matsuda
Report

Abstract

Establishment of cell lines representative of honeybee character would greatly assist in their analysis. Here, we show that immortalized cell line, designated as MYN9, has been generated from honeybee embryo by the gene transfer of human c-myc proto-oncogene. The morphology of the cell is characteristic of embryonic stem cell, although the cell is stable and does not spontaneously differentiate. Polymerase chain reaction analyses show that the cell is originated from authentic honeybee cell. It is proposed that the integration of human c-myc gene into honeybee precursor populations results in the establishment of stable cell line suitable for cellular and molecular studies.

Keywords

Honeybee Long-term culture c-myc Colony collapse disorder 

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Copyright information

© The Society for In Vitro Biology 2011

Authors and Affiliations

  • Yasuko Kitagishi
    • 1
  • Naoko Okumura
    • 1
  • Hitomi Yoshida
    • 1
  • Yuri Nishimura
    • 1
  • Jun-ichi Takahashi
    • 2
  • Satoru Matsuda
    • 1
  1. 1.Department of Environmental Health ScienceNara Women’s UniversityNaraJapan
  2. 2.Faculty of Life SciencesKyoto Sangyo UniversityKyotoJapan

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