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Establishment of clonal cell lines of taste buds from a p53 −/− mouse tongue

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Abstract

A taste bud is a sensory organ and consists of 50–100 spindle-shaped cells. The cells function as taste acceptors. They have characteristics of both epithelial and neuronal cells. A taste bud contains four types of cells, type I, type II, type III cells, and basal cells. Taste buds were isolated from a tongue of a p53-deficient mouse at day 12, and 11 clonal taste bud (TBD) cell lines were established. In immunochemical analysis, all cell lines expressed cytokeratin 18, gustducin, T1R3, and neural cellular adhesion molecule, but not GLAST. In RT-PCR analysis, shh was not expressed in any of the cell lines. Further analysis with RT-PCR was conducted on four cell lines. They expressed G protein-coupled taste receptors; T1R3, T2R8 for sweet, bitter, umami. And they also expressed α-ENaC for salty taste. While, a candidate for sour receptor HCN4 was expressed in TBD-a1 and TBD-a7 lines. And another candidate for sour receptor PKD1L3 was slightly expressed in TBD-a1 and TBD-c1.

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Acknowledgements

We would like to thank Dr. R. F. Malgorske (Mount Sinai School of Medicine) for providing anti-T1R3 antibody and Dr. S. Aizawa (RIKEN Center for Developmental Biology) for providing p53 −/− mice. Financial assistance was provided by KAKENHI (Grant 19570062), and by Program for Development of Strategic Research Center in Private Universities supported by MEXT (2010).

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Authors and Affiliations

  1. Department of Biological Science and Technology and Research Center for RNA Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Hideyuki Sako, Makie Hori, Atsumasa Okada & Yasuhiro Tomooka

  2. Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama-shi, Shiga, 526-0829, Japan

    Ikuo Masuho & Osamu Saitoh

Authors
  1. Hideyuki Sako
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  2. Makie Hori
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  3. Ikuo Masuho
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  4. Osamu Saitoh
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  5. Atsumasa Okada
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  6. Yasuhiro Tomooka
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Correspondence to Yasuhiro Tomooka.

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Editor: Tetsuji Okamoto

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Sako, H., Hori, M., Masuho, I. et al. Establishment of clonal cell lines of taste buds from a p53 −/− mouse tongue. In Vitro Cell.Dev.Biol.-Animal 47, 333–340 (2011). https://doi.org/10.1007/s11626-011-9398-3

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  • DOI: https://doi.org/10.1007/s11626-011-9398-3

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