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In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish

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Abstract

Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish skin and brain respectively, ZEB2J from zebrafish embryos, FHMT-W1 from fathead minnow testis, and Sf9 from ovaries of a fall armyworm moth. All cultures were maintained at 27°C. Infection was judged to have taken place by the appearance of sporonts and/or spores in cells and occurred in all cell lines. Spores were also isolated from ZEB2J cultures and used to successfully infect new cultures of ZEB2J, RK-13 and Sf9. These results suggest that cells of a wide range of vertebrates support A. algerae growth in vitro and fish cells can produce spores infectious to cells of mammals, fish, and insects

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Acknowledgments

The Natural Science and Engineering Research Council (NSERC) of Canada supported SRM through a postgraduate scholarship, and NCB and LEJL through Discovery Grants. The authors would also like to thank Aaron P. Frenette (PhD Candidate) and Dr. Michael S. Duffy at University New Brunswick for assistance with PCR authentication of A. algerae, and International Barcode of Life Project at University of Guelph for fish cell line authentication.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada, N2L 3G1

    S. Richelle Monaghan & Niels C. Bols

  2. Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada, N2L 3C5

    S. Richelle Monaghan, Rebecca L. Rumney, Nguyen T. K. Vo & Lucy E. J. Lee

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  1. S. Richelle Monaghan
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  2. Rebecca L. Rumney
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  3. Nguyen T. K. Vo
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  4. Niels C. Bols
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  5. Lucy E. J. Lee
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Correspondence to S. Richelle Monaghan.

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Editor: J. Denry Sato

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Monaghan, S.R., Rumney, R.L., Vo, N.T.K. et al. In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish. In Vitro Cell.Dev.Biol.-Animal 47, 104–113 (2011). https://doi.org/10.1007/s11626-010-9366-3

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  • DOI: https://doi.org/10.1007/s11626-010-9366-3

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