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Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio

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Abstract

The present study aims to evaluate the hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract on the carbon tetrachloride (CCl4)-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. H. sabdariffa extract (100, 200, and 400 μg/mL) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl4. CCl4 at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment and pre- and post-treatment of the hepatocytes with H. sabdariffa extract significantly reduced the elevated levels of LDH, GOT, GPT, and MDA and increased the reduced activities of SOD and GSH-Px in a dose-dependent manner; post-treatment did not show any protective effect. The results suggest that H. sabdariffa extract can be potentially used for preventing rather than curing liver diseases in fish.

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Acknowledgments

This study was funded by Ministry of Science and Technology of the People's Republic of China (2009DFA32620), co-funded by Department of Science and Technology of Jiangsu (BZ2008008), Wuxi Municipal Bureau on Science and Technology (CZE00906), and Central Public-Interest Scientific Institution Basal Research Fund (6-115009).

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Science, No. 9 East Shanshui Rd., Wuxi, 214081, China

    Guojun Yin, Liping Cao & Pao Xu

  2. Research Institute for Fisheries, Aquaculture and Irrigation, Anna light 8, Szarvas, 4440, Hungary

    Galina Jeney

  3. Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka, 812-8581, Japan

    Miki Nakao

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  1. Guojun Yin
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  2. Liping Cao
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  3. Pao Xu
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  4. Galina Jeney
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  5. Miki Nakao
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Correspondence to Guojun Yin.

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Editor: J. Denry Sato

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Yin, G., Cao, L., Xu, P. et al. Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio . In Vitro Cell.Dev.Biol.-Animal 47, 10–15 (2011). https://doi.org/10.1007/s11626-010-9359-2

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