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Derivation and characterization of three new human embryonic stem cell lines in Finland

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Human embryonic stem cell (hESC) lines can be established from the preimplantation embryos. Due to their ability to differentiate into all three embryonic layers, hESC are of significant interest as a renewable source of cell material for different applications, especially for cell replacement therapy. Since the establishment of the first hESC lines in 1998, several studies have described the derivation and culture of new hESC lines using various derivation methods and culture conditions. Our group has currently established eight new hESC lines of which three of the latest ones are described in a more detailed way in this report. The described lines have been established using mechanical derivation methods for surplus bad quality embryos and culture conditions containing human foreskin fibroblast feeder cells and serum-free culture medium. All the new lines have a normal karyotype and typical hESC characteristics analyzed in vitro. The described hESC lines are available for research purposes upon request (

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The hESC lines were cultured and characterized by Outi Melin, Hanna Koskenaho, Niina Ikonen, and Sari Leinonen. We also thank other Regea personnel for their support and especially Professor Riitta Suuronen. The personnel of the Tampere University Hospital’s Hormone and Infertility Clinic, Department of Obstetrics and Gynaecology Turku University Hospital and the tissue coordinators of Regea clinical tissue bank are acknowledged for their support in embryo donation procedures, Mrs. Jonna Rinne for revising the language and Ms. Heidi Hakala for assistance with figures.

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Correspondence to Heli Skottman.

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Editor: P. Andrews

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Skottman, H. Derivation and characterization of three new human embryonic stem cell lines in Finland. In Vitro Cell.Dev.Biol.-Animal 46, 206–209 (2010).

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