Abstract
The main purpose of this study was to investigate lysozyme synthesis and secretion in three human monocyte cell lines: U-937, HL-60, and THP-1, using sensitive fluorescence-based assay of lysozyme activity. PMA and hIFN-γ were evaluated for inducing lysozyme activity. Using well-defined cell lines from the cell culture collection, no lysozyme activity could be detected in the cultured U-937 cells either with or without addition of the inducing factors. These data suggested, contrary to previous reports, that U-937 cell line cannot synthesize or secrete active lysozyme. THP-1 and HL-60 cells were proved to produce enzymatically active lysozyme in increasing amounts with the time course. PMA and hIFN-γ had no significant inducing effect on the production or the release of active lysozyme in THP-1 and HL-60 cells. We showed inhibiting effect of PMA and hIFN-γ on the lysozyme activity, particularly in HL-60 cell line.
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Editor: J. Denry Sato
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Helal, R., Melzig, M.F. New aspects in the synthesis and secretion of lysozyme by cultured human monocyte cell lines. In Vitro Cell.Dev.Biol.-Animal 46, 492–496 (2010). https://doi.org/10.1007/s11626-010-9273-7
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DOI: https://doi.org/10.1007/s11626-010-9273-7