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Regulation of the expression balance of angiopoietin-1 and angiopoietin-2 by Shh and FGF-2

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Abstract

Sonic hedgehog (Shh) is a typical morphogen to regulate epithelial–mesenchymal interactions during embryonic development. Shh is also an indirect angiogenic agent upregulating other angiogenic factors, including angiopoietin-1 (Ang-1). Recent studies revealed that angiogenesis induced by Shh is characterized by distinct large-diameter vessels with less branching. Ang-1 promotes blood vessel maturation, and angiopoietin-2 (Ang-2) counteracts Ang-1 activity and regulates vascular branching. Thus, we hypothesized that Shh-induced angiogenesis is affected by expression of Ang-1 and Ang-2, and we investigated the regulatory system of angiopoietins by Shh in vitro. Shh enhanced Ang-1 expression but did not enhance vascular endothelial growth factor in fibroblasts. The upregulation of Ang-1 expression by Shh was significantly decreased by fibroblast growth factor-2 (FGF-2), a potent angiogenic factor. Furthermore, FGF-2 increased the expression of Ang-2 in endothelial cells. These findings suggest that Shh and FGF-2 regulate the expression balance of vascular morphogens Ang-1 and Ang-2 and are involved in angiogenesis.

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Acknowledgments

The authors would like to thank Saitoh Y, Yano T, Ohno M, Ida M, and Emura H for their secretarial assistance.

Competing Interest Statement

The authors declare that they have no competing financial interests.

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Authors and Affiliations

  1. Department of General Surgical Science, Graduate School of Medicine, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Takaaki Fujii & Hiroyuki Kuwano

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  1. Takaaki Fujii
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  2. Hiroyuki Kuwano
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Correspondence to Takaaki Fujii.

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Editor: J. Denry Sato

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Fujii, T., Kuwano, H. Regulation of the expression balance of angiopoietin-1 and angiopoietin-2 by Shh and FGF-2. In Vitro Cell.Dev.Biol.-Animal 46, 487–491 (2010). https://doi.org/10.1007/s11626-009-9270-x

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  • DOI: https://doi.org/10.1007/s11626-009-9270-x

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