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An effective method for the quantitative detection of porcine endogenous retrovirus in pig tissues

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Abstract

Xenotransplantation shows great promise for providing a virtually limitless supply of cells, tissues, and organs for a variety of therapeutical procedures. However, the potential of porcine endogenous retrovirus (PERV) as a human-tropic pathogen, particularly as a public health risk, is a major concern for xenotransplantation. This study focus on the detection of copy number in various tissues and organs in Banna Minipig Inbreed (BMI) from 2006 to 2007 in West China Hospital, Sichuan University. Real-time quantitative polymerase chain reaction (SYBR Green I) was performed in this study. The results showed that the pol gene had the most copy number in tissues compared with gag, envA, and envB. Our experiment will offer a rapid and accurate method for the detection of the copy number in various tissues and was especially suitable for the selection of tissues or organs in future clinical xenotransplantation.

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Acknowledgements

This research was supported by National Natural Science Foundation of China (Grant No. 30070725 and 30600677), National Basic Research Program of China (No.2003CB515504), and Program for Changjiang Scholars and Innovative Research Team in University, Ministry of Education.

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Authors and Affiliations

  1. Department of Urology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Peng Zhang

  2. Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Peng Zhang, Wei Wang, Li Zhang & Shengfu Li

  3. Laboratory of Cell and Gene Therapy, West China Second University Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Ping Yu

  4. Department of Pathology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Hong Bu

Authors
  1. Peng Zhang
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  2. Ping Yu
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  3. Wei Wang
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  4. Li Zhang
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  5. Shengfu Li
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  6. Hong Bu
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Corresponding author

Correspondence to Hong Bu.

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Editor: J. Denry Sato

Peng Zhang and Ping Yu contributed equally to this study.

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Zhang, P., Yu, P., Wang, W. et al. An effective method for the quantitative detection of porcine endogenous retrovirus in pig tissues. In Vitro Cell.Dev.Biol.-Animal 46, 408–410 (2010). https://doi.org/10.1007/s11626-009-9264-8

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  • DOI: https://doi.org/10.1007/s11626-009-9264-8

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