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Two new cell lines originated from the embryos of Clostera anachoreta (Lepidoptera: Notodontidae): characterization and susceptibility to baculoviruses

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Abstract

Two cell lines designated CAF-Clan I and CAF-Clan II have been established from embryos of Clostera anachoreta (Lepidoptera: Notodontidae) in TNM-FH medium containing 10% inactivated fetal bovine serum. CAF-Clan I consists of a mixture of three cell types: spherical cells, spindle-shaped cells, and giant cells. Most of the cultured cells formed a suspension in the medium and were subcultured more than 60 passages. CAF-Clan II mainly consists of spindle-shaped and spherical cells which attached to the culture surface and have undergone more than 40 passages. The cell population doubling time at 27°C of CAF-Clan I at passage 22 and CAF-Clan II at passage 24 was about 68.5 and 38.2 h, respectively. The chromosome number of both cell lines at passage 15 varied from 62 to 100 in the majority of cells, though a few cells exceeded 260 (n = 30). DNA amplification fingerprinting–polymerase chain reaction analysis confirmed that the origination of the two cell lines was C. anachoreta. The susceptibility of the cell lines to baculoviruses was tested. The results showed that CAF-Clan II was susceptible to infection of Autographa californica nucleopolyhedrovirus (AcMNPV) and Ecotropis oblique nucleopolyhedrovirus (EoNPV). Occlusion bodies (OBs) production was 129 ± 4 OBs/cell and 124 ± 15 OBs/cell for AcMNPV and EoNPV, respectively. CAF-Clan I was less susceptible to AcMNPV compared with CAF-Clan II, while non-permissive to EoNPV.

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Acknowledgments

This research was supported financially by National Natural Science Foundation of China (grant no. 30671688), by National Science and Technology Support Plan of China (grant no. 2006BAD08A1202), and by the State Forestry Administration Focus Project of China (grant no. 2005-9).

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Authors and Affiliations

  1. Key Laboratory of Forest Protection of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China

    Fayuan Wen, Yong-an Zhang, Liangjian Qu, Zhongqi Yang, Yuzhu Wang & Wenhuan Wang

  2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

    Huan Zhang & Qilian Qin

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  1. Fayuan Wen
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  2. Yong-an Zhang
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Correspondence to Yong-an Zhang.

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Editor: J. Denry Sato

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Wen, F., Zhang, Ya., Qu, L. et al. Two new cell lines originated from the embryos of Clostera anachoreta (Lepidoptera: Notodontidae): characterization and susceptibility to baculoviruses. In Vitro Cell.Dev.Biol.-Animal 45, 409–413 (2009). https://doi.org/10.1007/s11626-009-9196-3

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  • DOI: https://doi.org/10.1007/s11626-009-9196-3

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