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Pax2 overexpression in embryoid bodies induces upregulation of integrin α8 and aquaporin-1

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Abstract

The transcription factor Pax2 is essential for kidney development in mice, and overexpression of Pax2 in chick embryos leads to ectopic formation of nephric structures. We have generated embryonic stem (ES) cell lines that repress Pax2 expression in a tetracycline-dependent manner. In the absence of tetracycline, embryoid bodies derived from these cell lines expressed Pax2 and subsequently integrin α8 and aquaporin-1 (Aqp1), both of which are possibly involved in kidney development. Considering the slow induction kinetics, our data suggest that Pax2 and additional factors that are induced in embryoid bodies synergistically regulate the two targets. The ES cell lines with inducible Pax2 expression will also be useful for dissecting genetic cascades functioning in a variety of organ development.

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Acknowledgments

We thank H. Niwa for providing MGZRTcH2 cells. This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology and by the Ministry of the Health, Labor and Welfare of Japan.

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Authors and Affiliations

  1. Division of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan

    Akihiro Nakane & Ryuichi Nishinakamura

  2. Department of Nephro-urology, Graduate School of Medical Sciences, Nagoya City University, Nagoya City, Japan

    Akihiro Nakane, Yoshiyuki Kojima, Yutaro Hayashi & Kenjiro Kohri

  3. Division of Molecular Biology and Cell Engineering, Department of Regenerative Medicine, Research Institute, International Medical Center of Japan, Tokyo, Japan

    Shinji Masui

  4. Global COE Cell Fate Regulation Research and Education Unit, Kumamoto University, Kumamoto, Japan

    Ryuichi Nishinakamura

Authors
  1. Akihiro Nakane
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  2. Yoshiyuki Kojima
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  3. Yutaro Hayashi
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  4. Kenjiro Kohri
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  5. Shinji Masui
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  6. Ryuichi Nishinakamura
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Correspondence to Ryuichi Nishinakamura.

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Editor: J. Denry Sato

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Nakane, A., Kojima, Y., Hayashi, Y. et al. Pax2 overexpression in embryoid bodies induces upregulation of integrin α8 and aquaporin-1 . In Vitro Cell.Dev.Biol.-Animal 45, 62–68 (2009). https://doi.org/10.1007/s11626-008-9151-8

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  • DOI: https://doi.org/10.1007/s11626-008-9151-8

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