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Presence of side-population cells in an immortalized nontumorigenic human liver epithelial cell line

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Abstract

Side-population (SP) cells have been shown to be highly enriched stem cells. We investigated whether an immortalized, nontumorigenic human liver cell line, THLE-5b, contains SP cells. Flow cytometry analysis after Hoechst 33342 staining demonstrated that the THLE-5b line contained a small component of SP cells. These SP cells were essentially eliminated by treatment with verapamil and expressed higher levels of ABCG2 mRNA than non-SP cells. In addition, the level of these SP cells detected by Hoechst 33342 staining was affected by the experimental conditions including the incubation medium. This is the first report of the presence of SP cells in the immortalized, nontumorigenic human liver cell line.

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Acknowledgements

We thank Dr. CC Harris (National Cancer Institute, Bethesda, MD) for providing us with THLE-5b.

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Authors and Affiliations

  1. Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, 1-28-15 Kitashinagawa, Shinagawa, Tokyo, 140-0001, Japan

    Taisuke Yamazaki & Takayoshi Tokiwa

  2. Department of Regeneration Surgery, Research Institute, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan

    Shin Enosawa

  3. Kitashingawa Hospital, Kohno Clinical Medicine Research Institute, Shinagawa, Tokyo, 140-0001, Japan

    Takashi Tsukiyama

Authors
  1. Taisuke Yamazaki
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  2. Shin Enosawa
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  3. Takashi Tsukiyama
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  4. Takayoshi Tokiwa
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Corresponding author

Correspondence to Takayoshi Tokiwa.

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J. Denry Sato

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Yamazaki, T., Enosawa, S., Tsukiyama, T. et al. Presence of side-population cells in an immortalized nontumorigenic human liver epithelial cell line. In Vitro Cell.Dev.Biol.-Animal 44, 6–9 (2008). https://doi.org/10.1007/s11626-007-9071-z

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  • DOI: https://doi.org/10.1007/s11626-007-9071-z

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