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Ceramide kinase expression is altered during macrophage-like cell differentiation of the leukemia cell line HL-60

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Abstract

Ceramide kinase (CerK) has important roles in leukocyte functions, including the role in degranulation of mast cells and the phagocytosis of polymorphonuclear leukocytes, so its expression levels should be strictly regulated. Here, we report that the mRNA expression and enzyme activity of CerK were decreased during macrophage-like cell differentiation of the leukemia cell line HL-60, yet neither was altered during granulocytic differentiation of the same cells. Our findings demonstrate that HL-60 cells are useful for studying CerK functions in leukocyte differentiation, and they also suggest that CerK might have an important role in such differentiation.

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Authors and Affiliations

  1. Laboratory of Biomembrane and Biofunctional Chemistry, Faculty of Pharmaceutical Science and Faculty of Advanced Life Sciences, Hokkaido University, Sapporo, Japan

    Tomomi Date, Susumu Mitsutake & Yasuyuki Igarashi

  2. Department of Biomembrane and Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12. Nishi 6, Kita-ku, Sapporo, 060-0812, Japan

    Yasuyuki Igarashi

Authors
  1. Tomomi Date
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  2. Susumu Mitsutake
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  3. Yasuyuki Igarashi
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Correspondence to Yasuyuki Igarashi.

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Editor: J. Denry Sato.

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Date, T., Mitsutake, S. & Igarashi, Y. Ceramide kinase expression is altered during macrophage-like cell differentiation of the leukemia cell line HL-60. In Vitro Cell.Dev.Biol.-Animal 43, 321–323 (2007). https://doi.org/10.1007/s11626-007-9063-z

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  • DOI: https://doi.org/10.1007/s11626-007-9063-z

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