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Tissue Content and Pattern of Expression of Claudin-3 and Occludin in Normal and Neoplastic Tissues in Patients with Colorectal Cancer

  • Research Communication
  • Published:
Journal of Gastrointestinal Surgery

Abstract

Background

Metastasis is the worst prognostic variable of patients with colorectal cancer (CRC). For the development of metastases, it is necessary that cancer cells detach from the primary tumor, migrate into the angiolymphatic system, and invade the tissue where they will develop. The breakdown of the tight junctions (TJs) plays an important role in colorectal metastatic tumors. Claudin-3 and occludin are the main component proteins of TJs.

Aim

To analyze the expression and tissue content of claudin-3 and occludin in normal and neoplastic tissues of patients with metastatic CRC.

Methods

Fifty-seven consecutive patients with stage III and IV CRC were included. Fragments of neoplastic tissue were collected from the tumor margins, and samples of the normal tissue were collected from the same patient in a standardized distance of 10 cm from the cranial margin of the tumor. Immunohistochemistry technique was used to identify the tissue staining of claudin-3 and occludin. To measure the content of both proteins in cellular membranes of normal and cancer cells, a validated immunoscore was used.

Results

Claudin-3 and occludin in normal tissues are in the apical and lateral membranes of cells, while in the neoplastic, in cytoplasm. The mean of the tissue content of claudin-3 in the normal tissue was 2.57 ± 0.16, while in the neoplastic tissue was 1.03 ± 0.13. The contents of occludin were 2.77 ± 0.1 in normal tissue, while in the neoplastic were 1.08 ± 0.14.

Conclusion

There is a reduction in the content of the claudin-3 and occludin in the cell membranes of the neoplastic tissue in patients with CRC.

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Authors and Affiliations

  1. Post-Graduate Program in Healt Sciences, São Francisco University Medical School, Bragança Paulista, São Paulo, Brazil

    Roberta Laís Mendonça de Mattos, Danilo Toshio Kanno & Carlos Augusto Real Martinez

  2. Department of Gastroenterology, Faculty of Medicine, Universidade de São Paulo, São Paulo, Brazil

    Fábio Guilherme Campos

  3. Faculty of Medicine, São Francisco University Medical School, Bragança Paulista, São Paulo, Brazil

    Geovanna Pacciulli Pereira & Mateus Magami Yoshitani

  4. Post-Graduate Program in Sciences of Surgery, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil

    Andress de Godoy Delben

  5. Department of Pathology, São Francisco University Medical School, Bragança Paulista, São Paulo, Brazil

    José Aires Pereira

  6. Department of Surgery, Campinas State University, Campinas, Brazil

    Carlos Augusto Real Martinez

Authors
  1. Roberta Laís Mendonça de Mattos
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  2. Danilo Toshio Kanno
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  3. Fábio Guilherme Campos
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  4. Geovanna Pacciulli Pereira
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  5. Mateus Magami Yoshitani
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  6. Andress de Godoy Delben
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  7. José Aires Pereira
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  8. Carlos Augusto Real Martinez
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Corresponding author

Correspondence to Andress de Godoy Delben.

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Ethics Approval

The authors of the article “Tissue content and pattern of expression of claudin-3 and occludin in normal and neoplastic tissues in patients with colorectal cancer,” represented by the senior author Professor Carlos Augusto Real Martinez, would like to declare that the article was evaluated and approved by the Research Ethics Committee of Universidade São Francisco, Bragança Paulista, São Paulo, Brazil (process No 4.096.912).

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The authors declare no competing interests.

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de Mattos, R.L.M., Kanno, D.T., Campos, F.G. et al. Tissue Content and Pattern of Expression of Claudin-3 and Occludin in Normal and Neoplastic Tissues in Patients with Colorectal Cancer. J Gastrointest Surg 26, 2351–2353 (2022). https://doi.org/10.1007/s11605-022-05362-5

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  • DOI: https://doi.org/10.1007/s11605-022-05362-5

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