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Sponge Sampling with Fluorescent In Situ Hybridization as a Screening Tool for the Early Detection of Esophageal Cancer

  • 2016 SSAT Poster Presentation
  • Published:
Journal of Gastrointestinal Surgery Aims and scope

Abstract

Introduction

Sponge cytology is a novel screening tool for esophageal cancer but has been unable to be validated for widespread use. Our aim was to apply fluorescent in situ hybridization to sponge cytology samples in order to evaluate the safety and efficacy of this modality in screening for esophageal cancer.

Materials and Methods

At a single, multidisciplinary, NCI-designated cancer center, patients completed sponge cytology sampling prior to upper endoscopy. Samples were analyzed by p53 fluorescent in situ hybridization, and results were compared to the endoscopic diagnosis.

Results

Fifty patients were enrolled (96 % Caucasian, 68 % male, median age of 67). All patients successfully swallowed the capsule. No complications (string breakage, bleeding, mucosal injury) occurred. Endoscopy revealed that 38 % had normal esophageal mucosa and 62 % had an esophageal mucosal abnormality. In total, six samples demonstrated p53 loss (94 % specificity for any abnormality). The sensitivity of the p53 fluorescent in situ hybridization probe was13.3 % for any abnormality, 10 % for intestinal metaplasia, and 0 % for dysplasia or esophageal cancer.

Discussion

Esophageal sponge cytology is a promising, safe, and tolerable method for collecting esophageal cell samples. However, our data suggest that p53 fluorescent in situ hybridization does not improve the sensitivity for detecting cancer in these samples.

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Acknowledgments

We would like to acknowledge the contributions of Charlie Borzy, the study coordinator who maintains our data and was instrumental in study design and IRB approval. We additionally thank Mary Kwatkosky-Lawlor for her assistance with the manuscript editing and formatting.

Authorship

All authors listed above have contributed substantially to the design and acquisition of the data in this study as well as drafting of and final approval of this manuscript.

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Authors and Affiliations

  1. Division of Gastrointestinal and General Surgery, Department of Surgery, Oregon Health and Science University, 3181 SW Sam Jackson Pk Rd, L223A, Portland, OR, 97239, USA

    Kelly R. Haisley MD, James P. Dolan MD, MCR, Sergio A. Toledo-Valdovinos MD, Kyle D. Hart MS & John G. Hunter MD

  2. Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, USA

    Susan B. Olson PhD

  3. Department of Gastroenterology, Oregon Health and Science University, Portland, OR, USA

    Gene Bakis MD & Brintha K. Enestvedt MD

Authors
  1. Kelly R. Haisley MD
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  2. James P. Dolan MD, MCR
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  3. Susan B. Olson PhD
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  4. Sergio A. Toledo-Valdovinos MD
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  5. Kyle D. Hart MS
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  6. Gene Bakis MD
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  7. Brintha K. Enestvedt MD
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  8. John G. Hunter MD
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Corresponding author

Correspondence to John G. Hunter MD.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Funding Sources

This study was funded by the Newton Foundation Grant.

Additional information

Meeting Presentation

Digestive Diseases Week, May 21–24, 2016. San Diego, CA (Poster)

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Haisley, K.R., Dolan, J.P., Olson, S.B. et al. Sponge Sampling with Fluorescent In Situ Hybridization as a Screening Tool for the Early Detection of Esophageal Cancer. J Gastrointest Surg 21, 215–221 (2017). https://doi.org/10.1007/s11605-016-3239-3

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  • DOI: https://doi.org/10.1007/s11605-016-3239-3

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