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Ischemic Preconditioning-Like Effect of Polyunsaturated Fatty Acid-Rich Diet on Hepatic Ischemia/Reperfusion Injury

  • 2011 SSAT Poster Presentation
  • Published:
Journal of Gastrointestinal Surgery

Abstract

Aim

The aim of this study was to investigate a possible preconditioning effect of oral diet enriched with polyunsaturated fatty acids (PUFAs) on liver ischemia/reperfusion (I/R) injuries.

Methods

Wistar male rats were fed a standard diet or polyunsaturated fatty acid-rich diet (PRD) enriched with (GII) or without (GIII) ω-3 PUFA. Rats were submitted to partial liver ischemia during 1 h and evaluated in pre- and post-I/R conditions. In pre-I/R condition, livers were collected for determination of fatty acid composition, liver mitochondrial function, malondialdehyde (MDA) content, and histological analysis. Four hours after liver reperfusion serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), serum levels of tumor necrosis factor-alpha, interleukin-6, interleukin-10, and prostaglandin-E2, liver mitochondrial function, MDA content, and histology were evaluated.

Results

In the pre-I/R condition, GII and GIII groups had an increase on PUFA content and exhibited slight increased macrosteatosis and microsteatosis in the liver. After 4 h of reperfusion, PRD-fed rats showed a marked decrease on steatosis, diminished necrosis, an increase in MDA formation, and mitochondrial uncoupling. We also observed a marked decrease in plasma levels of cytokines and ALT and AST activities in post-I/R condition in PRD groups.

Conclusion

In this experimental model in the rat, PRD has a preconditioning effect protecting the liver from I/R injury and should be object of future clinical studies.

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Acknowledgments

The authors are indebted to the technical assistance of Nilza Molan and Alcione Lescano.

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Authors and Affiliations

  1. Department of Gastroenterology (LIM/37-LIM/07), Medical School, University of Sao Paulo, R. Peixoto Gomide, 515 13 andar, 01409001, Sao Paulo, Sao Paulo, Brazil

    Ana Maria Mendonça Coelho, Marcel Cerqueira Cesar Machado, Sandra N Sampietre, José Tadeu Stefano, Andre Zonetti A. Leite & Luiz A. Carneiro D’Albuquerque

  2. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

    Hilton Kenji Takahashi & Rui Curi

Authors
  1. Ana Maria Mendonça Coelho
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  2. Marcel Cerqueira Cesar Machado
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  8. Luiz A. Carneiro D’Albuquerque
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Corresponding author

Correspondence to Marcel Cerqueira Cesar Machado.

Additional information

This work was presented at the 52nd Annual Meeting of the Society for Surgery of the Alimentary Tract on May 2011, in Chicago, IL.

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Coelho, A.M.M., Machado, M.C.C., Takahashi, H.K. et al. Ischemic Preconditioning-Like Effect of Polyunsaturated Fatty Acid-Rich Diet on Hepatic Ischemia/Reperfusion Injury. J Gastrointest Surg 15, 1679–1688 (2011). https://doi.org/10.1007/s11605-011-1648-x

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  • DOI: https://doi.org/10.1007/s11605-011-1648-x

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