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PinX1 Inhibits Telomerase Activity in Gastric Cancer Cells Through Mad1/c-Myc Pathway

  • Original Article
  • Published:
Journal of Gastrointestinal Surgery

Abstract

Introduction

The aim of this study was to investigate the role of Mad1/c-Myc in telomerase regulation in gastric cancer cells in order to gain insight into telomerase activity and to evaluate PinX1 as a putative inhibitor of gastric cancer.

Methods

PinX1 and PinX1siRNA eukaryotic expression vectors were constructed by recombinant technology and transfected into gastric carcinoma cells using Lipofectamine™ 2000. Telomerase activity was measured by the telomeric repeat amplification protocol. Apoptosis of gastric cancer cells was analyzed by flow cytometry and transmission electron microscopy. Reverse transcription-polymerase chain reaction and Western blotting were used to assess the expression levels of PinX1 and Mad1/c-Myc.

Results

We found that PinX1-negative gastric cancer cells showed significantly higher telomerase activity than did the PinX1-postive cells. PinX1-transfection reduced telomerase activity in PinX1-negative gastric cancer cells and exhibited an upregulation of Mad1 and downregulation of c-Myc expression. Pinx1 RNAi treatment led to downregulation of Mad1 and upregulation of c-Myc.

Conclusion

Suppression of telomerase activity mediated by PinX1 is involved in the Mad1/c-Myc pathway.

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Authors and Affiliations

  1. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

    Hong-bin Wang, Xing-wei Wang, Gang Zhou, Wei-qiang Wang, Yong-gang Sun, Shi-ming Yang & Dian-chun Fang

  2. 324 Hospital of PLA, Chongqing, China

    Hong-bin Wang

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  1. Hong-bin Wang
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  2. Xing-wei Wang
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  3. Gang Zhou
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Correspondence to Dian-chun Fang.

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Wang, Hb., Wang, Xw., Zhou, G. et al. PinX1 Inhibits Telomerase Activity in Gastric Cancer Cells Through Mad1/c-Myc Pathway. J Gastrointest Surg 14, 1227–1234 (2010). https://doi.org/10.1007/s11605-010-1253-4

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  • DOI: https://doi.org/10.1007/s11605-010-1253-4

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