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Immunohistochemical Expression of Osteopontin in Gastric Cancer

  • Original Article
  • Published:
Journal of Gastrointestinal Surgery

Abstract

Background/Aims

Osteopontin (OPN) is significantly overexpressed in a variety of malignancies. However, little is known concerning the significance of OPN expression in human cancers. Thus, the aim of this study was to determine the relationship between the degree of OPN expression, the proliferative activity of cancer cells, and the clinicopathological findings for surgically resected gastric cancer.

Methodology

We evaluated the immunohistochemical expression of OPN in 85 specimens of cancer. Additionally, we investigated a cancer cell proliferative index using an anti-MIB-1 antibody and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling staining. Levels of OPN expression in gastric cancers were classified into three groups. To compare the relationship between OPN expression and clinicopathological findings, the features of cancer lesions were classified using the TNM Classification of Malignant Tumors, 6th Edition.

Results

Immunohistochemical examination of OPN expression in gastric cancer revealed diffuse granular staining in the cytoplasm. High OPN expression was observed in 37 of 85 carcinomas. Strong OPN expression was significantly associated with a low apoptotic index, a high proliferative index, depth of invasion, lymphatic invasion, and venous invasion. Pathologically, intestinal type carcinoma showed strong expression of OPN.

Conclusions

These data suggested that OPN may play an important role in the invasiveness and the progressive nature of gastric cancer.

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Authors and Affiliations

  1. Department of Surgery, Kinki University School of Medicine, 377–2 Ohno-higashi, Osaka-Sayama, Osaka, 589–8511, Japan

    Motohiro Imano, Kenichi Sakai, Eizaburo Ishimaru, Atsushi Yasuda, Ying-Feng Peng, Masayuki Shinkai, Takushi Yasuda, Haruhiko Imamoto, Kiyokata Okuno, Hitoshi Shiozaki & Harumasa Ohyanagi

  2. Ambulatory Treatment Center, Kinki University School of Medicine, 377–2 Ohno-higashi, Osaka-Sayama, Osaka, 589–8511, Japan

    Motohiro Imano & Kiyokata Okuno

  3. Pathology, Kinki University School of Medicine, 377–2 Ohno-higashi, Osaka-Sayama, Osaka, 589–8511, Japan

    Takao Satou, Tatsuki Itoh & Hiroyuki Ito

  4. Neurosurgery, Kinki University School of Medicine, 377–2 Ohno-higashi, Osaka-Sayama, Osaka, 589–8511, Japan

    Fumiharu Akai

Authors
  1. Motohiro Imano
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  2. Takao Satou
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  3. Tatsuki Itoh
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  4. Kenichi Sakai
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  5. Eizaburo Ishimaru
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  7. Ying-Feng Peng
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  9. Fumiharu Akai
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  13. Hiroyuki Ito
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Correspondence to Motohiro Imano.

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Imano, M., Satou, T., Itoh, T. et al. Immunohistochemical Expression of Osteopontin in Gastric Cancer. J Gastrointest Surg 13, 1577–1582 (2009). https://doi.org/10.1007/s11605-009-0955-y

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  • DOI: https://doi.org/10.1007/s11605-009-0955-y

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