Skip to main content

Advertisement

SpringerLink
Log in

Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm

  • original article
  • Published:
Journal of Gastrointestinal Surgery

Abstract

Background

Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice.

Aim

The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel.

Methods

Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6 am–6 pm) were sacrificed at 9 am, 3 pm, 9 pm, and 3 am (n = 6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9 am and 9 pm.

Results

mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (p ≤ 0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (p < 0.05) but not in ileum. Transporter-mediated glucose uptake was greater at 9 pm than 9 am (p ≤ 0.04) in all three segments. V max was greater in duodenum (10 vs 6 nmol/cm/s) and jejunum (8 vs 5 nmol/cm/s) at 9 pm compared to 9 am (p = 0.01); K m remained unchanged.

Summary

mRNA levels of intestinal hexose transporters varied diurnally. Protein levels peaked 6–12 h later during dark cycle when >70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9 pm.

Conclusion

Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  1. Affleck JA, Helliwell PA, Kellett GL. Immunocytochemical detection of GLUT2 at the rat intestinal brush-border membrane. J Histochem Cytochem 2003;51:1567–1574.

    PubMed  Google Scholar 

  2. Au A, Gupta A, Schembri P, Cheeseman CI. Rapid insertion of GLUT2 into the rat jejunal brush-border membrane promoted by glucagon-like peptide. Biochem J 2002;367:247–254. doi:10.1042/BJ20020393.

    Article  PubMed  Google Scholar 

  3. Bell GI, Burant CF, Takeda J, Gould GW. Structure and function of mammalian facilitative sugar transporters. J Biol Chem 1993;268:19161–19164.

    PubMed  Google Scholar 

  4. Castello A, Guma A, Sevilla L, Furriols M, Testar X, Palacin M, et al. Regulation of GLUT5 gene expression in rat intestinal mucosa: regional distribution, circadian rhythm, perinatal development and effect of diabetes. Biochem J 1995;309(Pt 1):271–277.

    PubMed  Google Scholar 

  5. Corpe CP, Bovelander FJ, Hoekstra JH, Burant CF. The small intestinal fructose transporters: site of dietary perception and evidence for diurnal and fructose sensitive control elements. Biochim Biophys Acta 1998;1998(1402):229–238.

    Google Scholar 

  6. Corpe CP, Burant CF, Hoekstra JH. Intestinal fructose absorption: clinical and molecular aspects. J Pediatr Gastroenterol Nutr 1999;28:364–374. doi:10.1097/00005176-199904000-00004.

    Article  PubMed  Google Scholar 

  7. Corpe CP, Burant CF. Hexose transporter expression in rat small intestine: effect of diet on diurnal variations. Am J Physiol 1996;271:G211–G216.

    PubMed  Google Scholar 

  8. Helliwell PA, Richardson M, Affleck J, Kellett GL. Stimulation of fructose transport across intestinal brush-border membrane by PMA is mediated by GLUT2 and dynamically regulated by protein kinase C. Biochem J 2000;350:149–154. doi:10.1042/0264-6021:3500149.

    Article  PubMed  Google Scholar 

  9. Helmrath MA, VanderKolk WE, Can G, Erwin CR, Warner BW. Intestinal adaptation following massive small bowel resection in the mouse. J Am Coll Surg 1996;183:441–449.

    PubMed  Google Scholar 

  10. Houghton SG, Duenes JA, Fatima J, Iqbal CW, Kasparek MS, Sarr MG. Coordinated, diurnal hexose transporter expression in rat small bowel: implications for small bowel resection. Surgery 2008;143:79–93. doi:10.1016/j.surg.2007.06.007.

    Article  PubMed  Google Scholar 

  11. Houghton SG, Zarroug AE, Duenes JA, Fernandez-Zapico ME, Sarr MG. The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation. Surgery 2006;139:542–549. doi:10.1016/j.surg.2005.09.002.

    Article  PubMed  Google Scholar 

  12. Karasov WH, Diamond JM. A simple method for measuring intestinal solute uptake in vitro. J Comp Physiol 1983;152:105–116.

    Google Scholar 

  13. Levin RJ. Digestion and absorption of carbohydrates—from molecules and membranes to humans. Am J Clin Nutr 1994;59:690S–698S.

    PubMed  Google Scholar 

  14. Kellett GL. The facilitated component of intestinal glucose absorption. J Physiol 2001;531:585–595. doi:10.1111/j.1469-7793.2001.0585h.x.

    Article  PubMed  Google Scholar 

  15. Kellet GL, Helliwell PA. The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush border. Biochem J 2000;350:155–162. doi:10.1042/0264-6021:3500155.

    Article  Google Scholar 

  16. Kipp H, Khoursandi S, Scharlau D, Kinne RK. More than apical: distribution of SGLT1 in CaCO-2 cells. Am J Physiol 2003;285:C737–C749.

    Google Scholar 

  17. Pan X, Terada T, Okuda M, Inui K. The diurnal rhythm of the intestinal transporters SGLT1 and PEPT1 is regulated by the feeding conditions in rats. J Nutr 2004;134:2211–2215.

    PubMed  Google Scholar 

  18. Rhoads DB, Rosenbaum DH, Unsal H, Isselbacher KJ, Levitsky LL. Circadian periodicity of intestinal Na+/glucose cotransporter 1 mRNA levels is transcriptionally regulated. J Biol Chem 1998;273:9510–9516. doi:10.1074/jbc.273.16.9510.

    Article  PubMed  Google Scholar 

  19. Tavakkolizadeh A, Berger UV, Shen KR, Levitsky LL, Zinner MJ, Hediger MA, et al. Whang EEl. Diurnal rhythmicity in intestinal SGLT-1 function, V(max), and mRNA expression topography. Am J Physiol 2001;280:G209–G215.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Gastroenterology Research Unit (GU 1001), Department of Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA

    Javairiah Fatima, Corey W. Iqbal, Scott G. Houghton, Michael S. Kasparek, Judith A. Duenes, Ye Zheng & Michael G. Sarr

Authors
  1. Javairiah Fatima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Corey W. Iqbal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Scott G. Houghton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael S. Kasparek
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Judith A. Duenes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ye Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Michael G. Sarr
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael G. Sarr.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fatima, J., Iqbal, C.W., Houghton, S.G. et al. Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm. J Gastrointest Surg 13, 634–641 (2009). https://doi.org/10.1007/s11605-008-0776-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11605-008-0776-4

Keywords

Search

Navigation