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Negatively charged superparamagnetic iron oxide nanoparticles: a new blood-pooling magnetic resonance contrast agent

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Japanese Journal of Radiology Aims and scope Submit manuscript

Abstract

Purpose

Our purpose was to investigate the utility of superparamagnetic iron-oxide nanoparticles (SPIO) as a blood-pooling contrast agent at magnetic resonance imaging (MRI).

Materials and methods

We studied four contrast agents: carboxymethyl-diethylaminoethyl dextran magnetite SPIO (CMEADM-S, diameter 54 nm), negatively charged CMEADM ultrasmall SPIO (CMEADM-U, 32 nm), alkali-treated dextran magnetite SPIO (ATDM-S, 55 nm), and ATDM ultrasmall SPIO (ATDM-U, 28 nm) carrying a neutral charge. Each contrast agent (80 μmol/kg) was injected intraperitoneally into apolipoprotein E (apoE) mice and the tissue iron concentration was measured 30-, 60-, 180-, and 300-min later by nuclear MR. For MR angiographic (MRA) evaluation, we injected the agents into the auricular vein of four groups of 15 rabbits. Immediately and 30-, 60-, 180-, and 300-min later, three rabbits from each group were subjected to MRI. The organ/background signal ratio (SR) was calculated. Statistical analyses were performed with Tukey’s honestly significant difference (HSD) test.

Results

At 60 and 180 min, blood-iron concentration of CMEADM-U was significantly different from other contrast agents. In the abdominal aorta and inferior vena cava, SR of CMEADM-U was higher at 180 and 300 min than of the other contrast agents. In the thoracic aorta, there was no difference in SR at 300 min between CMEADM-U and CMEADM-S.

Conclusion

Negatively charged SPIO nanoparticles may be useful as a blood-pooling contrast agent.

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Authors and Affiliations

  1. Department of Radiology, Shiga University of Medical Science, Tsukinowaco, Seta, Otsu, Shiga, 520-2192, Japan

    Norihisa Nitta, Keiko Tsuchiya, Akinaga Sonoda, Shinichi Ota, Noritoshi Ushio, Masashi Takahashi & Kiyoshi Murata

  2. The Nagoya Research Laboratory, Meito Sangyo Co., Ltd., 25-5 Nishibiwajima-cho, Kiyosu, Aichi, 452-0067, Japan

    Satoshi Nohara

Authors
  1. Norihisa Nitta
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  2. Keiko Tsuchiya
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  3. Akinaga Sonoda
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  4. Shinichi Ota
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  5. Noritoshi Ushio
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  6. Masashi Takahashi
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  7. Kiyoshi Murata
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  8. Satoshi Nohara
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Correspondence to Norihisa Nitta.

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Nitta, N., Tsuchiya, K., Sonoda, A. et al. Negatively charged superparamagnetic iron oxide nanoparticles: a new blood-pooling magnetic resonance contrast agent. Jpn J Radiol 30, 832–839 (2012). https://doi.org/10.1007/s11604-012-0133-0

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  • DOI: https://doi.org/10.1007/s11604-012-0133-0

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