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Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique

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Abstract

Purpose

The aim of this study was to select a suitable substrate candidate for dynamic nuclear polarization (DNP) studies and demonstrate its utility for evaluating intracellular metabolism.

Materials and methods

Hyperpolarized substances included 1-13C-pyruvate (Pyr), 1-13C-glucose (Glc), and 1-13C-acetate. A DNP polarizer and a 600-MHz vertical small-bore scanner were used for 13C-MR spectroscopic measurements. After polarization for 1 h, the dissolved solution was injected via a capillary line into the nuclear magnetic resonance tube in the scanner. The sequential spectra of the hyperpolarized 13C-labeled substrates were acquired in durations of more than 120 s, and a thermal spectrum was obtained more than 1 h thereafter. FM3A cancer cells of mammary tumors were cultured for intracellular detection of the hyperpolarized 13C-substances.

Results

The greatest sensitivity was found using Pyr with the longest T1 decay (51.5 s); and remarkably, the least sensitivity was observed using Glc with a signal decay of less than 2 s. An effective increase in sensitivity was shown using the other substances. The hyperpolarized intracellular study using 13C-Pyr showed distinct elevation of lactate levels.

Conclusion

The DNP technique is useful for evaluating intracellular metabolism. However, Glc is not suitable for use with the DNP technique.

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Authors and Affiliations

  1. Department of Medical Imaging, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15 Kuramoto-Cho, Tokushima, 770-8509, Japan

    Masafumi Harada & Hitoshi Kubo

  2. MRI/Biotools Division, Oxford Instruments KK, Tokyo, Japan

    Takamasa Abe

  3. Department of Radiation Physics, Engineering, and Biology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan

    Hiroshi Maezawa

  4. Department of Radiology, Institute of Health Biosciences, University of Tokushima, Tokushima, Japan

    Hideki Otsuka

Authors
  1. Masafumi Harada
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  2. Hitoshi Kubo
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  3. Takamasa Abe
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  4. Hiroshi Maezawa
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  5. Hideki Otsuka
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Corresponding author

Correspondence to Masafumi Harada.

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Harada, M., Kubo, H., Abe, T. et al. Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique. Jpn J Radiol 28, 173–179 (2010). https://doi.org/10.1007/s11604-009-0390-8

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  • DOI: https://doi.org/10.1007/s11604-009-0390-8

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