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Responses of total and quiescent cell populations in solid tumors to carbon ion beam irradiation (290 MeV/u) in vivo

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Abstract

Purpose

The aim of this study was to clarify the radiosensitivity of intratumor total cells and quiescent (Q) cells in vivo to accelerated carbon ion beams compared with γ-ray irradiation.

Materials and methods

Squamous cell carcinoma (SCC) VII tumor-bearing mice received continuous administration of 5-bromo-2′-deoxyuridine (BrdU) to label all intratumor proliferating (P) cells. They then were exposed to carbon ions (290 MeV/u) or γ-rays. Immediately after and 12 h after irradiation, immunofluorescence staining for BrdU was used to assess the response of Q cells in terms of micronucleus frequency. The response of the total (P + Q) tumor cells was determined from the tumors not treated with BrdU.

Results

The apparent difference in radiosensitivity between total and Q cell populations under γ-ray irradiation was markedly reduced with carbon ion beams, especially with a higher linear energy transfer (LET) value. Clearer recovery in Q cells than in total cells through delayed assay under γ-ray irradiation was efficiently inhibited by carbon ion beams, especially those with a higher LET.

Conclusion

In terms of the tumor cell-killing effect as a whole, including intratumor Q cells, carbon ion beams, especially with higher LET values, were extremely useful for suppressing the dependence on the heterogeneity within solid tumors as well as depositing the radiation dose precisely.

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Authors and Affiliations

  1. Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University, 2-1010 Asashiro-nishi, Kumatori-cho, Sennan, Osaka, 590-0494, Japan

    Shin-ichiro Masunaga & Koji Ono

  2. Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Japan

    Koichi Ando, Akiko Uzawa, Ryoichi Hirayama, Yoshiya Furusawa & Sachiko Koike

Authors
  1. Shin-ichiro Masunaga
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  2. Koichi Ando
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  3. Akiko Uzawa
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  4. Ryoichi Hirayama
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  5. Yoshiya Furusawa
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  6. Sachiko Koike
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  7. Koji Ono
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Correspondence to Shin-ichiro Masunaga.

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Masunaga, Si., Ando, K., Uzawa, A. et al. Responses of total and quiescent cell populations in solid tumors to carbon ion beam irradiation (290 MeV/u) in vivo. Radiat Med 26, 270–277 (2008). https://doi.org/10.1007/s11604-008-0227-x

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  • DOI: https://doi.org/10.1007/s11604-008-0227-x

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