Abstract
Objective
Population genetic analysis based on genetic markers harbors valuable forensic applications. In this regard, it is informative and imperative to explore Han groups as they are the largest population of China. In particular, there is a largely underrepresented amount of information from recent decades regarding the southeast costal Han Chinese. Therefore, the aim of this study is to investigate the available genetic characteristics of the Han population living in the Jinjiang, Fujian Province, Southeastern China.
Methods
We sampled 858 saliva samples and used the commercially available Microreader™ Y Prime Plus ID System to identify population data of Y-short tandem repeat (STR) loci of this region.
Results
A total of 822 different haplotypes were observed. The overall haplotype diversity, discriminatory power and haplotype match probability were 0.9999, 0.9999 and 0.0012, respectively.
Conclusion
Our results showed that the Jinjiang Han population was closely genetically related to Han groups of China. Overall, we identified a set of 37 Y-STRs that are highly polymorphic, and that can provide meaningful information in forensic practice and human genetic research.
Similar content being viewed by others
References
Roewer L. Y chromosome STR typing in crime casework. Forensic Sci Med Pathol, 2009,5(2):77–84
Qian XQ, Hou JY, Wang Z, et al. Next Generation Sequencing Plus (NGS+) with Y-chromosomal Markers for Forensic Pedigree Searches. Sci Rep, 2017,7(1):11324
Purps J, Geppert M, Nagy M, et al. Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases. Forensic Sci Int Genet, 2015,19:238–242
Corach D, Lao O, Bobillo C, et al. Inferring Continental Ancestry of Argentineans from Autosomal, Y-Chromosomal and Mitochondrial DNA. Ann Hum Genet, 2010,74(1):65–76
Liu J, Ming TY, Lang M, et al. Exploitation of a novel slowly mutating Y-STRs set and evaluation of slowly mutating Y-STRs plus Y-SNPs typing strategy in forensic genetics and evolutionary research. Electrophoresis, 2021,42(6):774–785
Nothnagel M, Fan GY, Guo F, et al. Revisiting the male genetic landscape of China: a multi-center study of almost 38,000 Y-STR haplotypes. Hum Genet, 2017,136(5):485–497
Lang M, Liu H, Song F, et al. Forensic characteristics and genetic analysis of both 27 Y-STRs and 143 Y-SNPs in Eastern Han Chinese population. Forensic Sci Int Genet, 2019,42(7):e13–e20
Wang Z, Du WA, He GL, et al. Forensic characteristics and phylogenetic analysis of Hubei Han population in central China using 17 Y-STR loci. Forensic Sci Int Genet, 2017,29:e4–e8
Wang MG, Wang Z, Zhang YQ, et al. Forensic characteristics and phylogenetic analysis of two Han populations from the southern coastal regions of China using 27 Y-STR loci. Forensic Sci Int Genet, 2017,31:e17–e23
Li LY, Yao LL, He X, et al. Haplotype diversity and phylogenetic characteristics for Guanzhong Han population from Northwest China via 38 Y-STRs using Yfiler™ Platinum Amplification System. Mol Genet Genomic Med, 2020,8(5):e1187
Huang YM, Guo LW, Wang MM, et al. Genetic analysis of 39 Y-STR loci in a Han population from Henan province, central China. Int J Legal Med, 2019,133(1):95–97
Zhao Y, Yao X, Li Y, et al. Developmental validation of the Microreader Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications. Sci Justice, 2021,61(3):260–270
Zhao Y, Zhang J, He T, et al. Validation of the Microreader 29Y Prime ID system for forensic use. Forensic Sci Int, 2020,309:110147
Gusmão L, Butler JM, Carracedo A, et al. DNA Commission of the International Society of Forensic Genetics (ISFG): An update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Sci Int Genet, 2016,157(2–3):187–197
Roewer L, Andersen MM, Ballantyne J, et al. DNA commission of the International Society of Forensic Genetics (ISFG): Recommendations on the interpretation of Y-STR results in forensic analysis. Forensic Sci Int Genet, 2020,48(7):102308
Nei M, Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics, 1981,97(1):145–163
Purps J, Siegert S, Willuweit S, et al. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Sci Int Genet, 2014,12(100):12–23
Meirmans PG. Amova-based clustering of population genetic data. J Hered, 2012,103(5):744–750
Zhou YS, Fang YT, Jin XY, et al. Haplotype diversity and phylogenetic relationship analysis of Chinese Yulin Han population using 59 Y-STR loci of two novel Y-STR typing systems. Leg Med (Tokyo), 2021,17(50):101871
Acknowledgements
We here thank Xin HE for the help of data analysis.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
No potential conflict of interest is reported by the authors.
Additional information
This study was supported by the Shaanxi Basic Research Program of Natural Science (No. 2021JQ-392).
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Li, Ly., Wu, Dq., Yao, Ll. et al. Improving DNA Data Capacity: Forensic Parameters and Genetic Structure Analysis of Jinjiang Han Population with the Microreader™ Y Prime Plus ID System. CURR MED SCI 42, 462–466 (2022). https://doi.org/10.1007/s11596-022-2570-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-022-2570-z