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Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells

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Summary

Activation of acid-sensing ion channels (ASICs) plays an important role in neuroinflammation. Macrophage recruitment to the sites of inflammation is an essential step in host defense. ASIC1 and ASIC3 have been reported to mediate the endocytosis and maturation of bone marrow derived macrophages. However, the expression and inflammation-related functions of ASICs in RAW 264.7 cells, another common macrophage, are still elusive. In the present study, we first demonstrated the presence of ASIC1, ASIC2a and ASIC3 in RAW 264.7 macrophage cell line by using reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence experiments. The non-specific ASICs inhibitor amiloride and specific homomeric ASICla blocker PcTxl reduced the production of iNOS and COX-2 by LPS-induced activating RAW 264.7 cells. Furthermore, not only amiloride but also PcTxl inhibited the migration and LPS-induced apoptosis of RAW 264.7 cells. Taken together, our findings suggest that ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells, and ASICs may serve as a potential novel target for immunological disease therapy.

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Authors and Affiliations

  1. Department of Pharmacology, Huazhong University of Science and Technology, Wuhan, 430030, China

    Lan Ni, Peng Fang, Zhuang-li Hu, Hai-yun Zhou, Jian-guo Chen, Fang Wang & You Jin

  2. Key Laboratory of Neurological Diseases (HUST), Ministry of Education of China, Huazhong University of Science and Technology, Wuhan, 430030, China

    Lan Ni, Zhuang-li Hu, Jian-guo Chen, Fang Wang & You Jin

  3. Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province, Huazhong University of Science and Technology, Wuhan, 430030, China

    Lan Ni, Zhuang-li Hu, Jian-guo Chen, Fang Wang & You Jin

  4. Institute of Brain Research, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Jian-guo Chen & Fang Wang

Authors
  1. Lan Ni
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  2. Peng Fang
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  3. Zhuang-li Hu
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  4. Hai-yun Zhou
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  5. Jian-guo Chen
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  6. Fang Wang
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  7. You Jin
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Correspondence to You Jin.

Additional information

This work was snpported by grants from the National Natural Science Foundalion of China (No. 81473199), and the Fundamental Researcb Funds for the Central Universities (No. 015TS125).

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Ni, L., Fang, P., Hu, Zl. et al. Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells. CURR MED SCI 38, 436–442 (2018). https://doi.org/10.1007/s11596-018-1897-y

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  • DOI: https://doi.org/10.1007/s11596-018-1897-y

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