Skip to main content
Log in

Measurement Uncertainty Assessment for Determination of Allura Red in Puffed Food by HPLC

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

This study aimed to assess the measurement uncertainty of a new method for determination of allura redin food by high performance liquid chromatography (HPLC). The uncertainty of mathematical model of allura red is based on Europe for Analytical Chemistry(EURACHEM) guidelines. The sources and components of uncertainty were calculated, including recovery, working solution, sample mass, final volume, response of standard solution, response of sample solution. The expanded uncertainty was 0.0024 (k=2). Uncertainty of working solution was the most significant factor contributing to the total uncertainty, accounting for 86.2%. The uncertainty of volume accounted for the minimum at 0.025%. The developed method is simple and accurate, which can be used for the determination of allura redin puffed samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Pourreza N, Rastegarzadeh S, Larki A. Determination of Allura red in food samples after cloud point extraction using mixed micelles. Food Chemistry, 2011,126(3):1465–1469

    Article  CAS  Google Scholar 

  2. Yu Y, Fan Z. Magnetic solid-phase extraction coupled with HPLC for the determination of Allura Red in food and beverage samples. Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2016,33(10):1527–1534

    Article  PubMed  CAS  Google Scholar 

  3. Shi Y, Li C, Liu S, et al. A novel method for detecting allura red based on triple-wavelength overlapping resonance Rayleigh scattering, Rsc Advances, 2014,4(70):37100–37106

    Article  CAS  Google Scholar 

  4. Zhang J, Zhang S, Wang X, et al. Simultaneous determination of Ponceau-4R and Allura Red in soft drinks based on the ionic liquid modified expanded graphite paste electrode. Int J Environ An Ch, 2015,95(7):581–591

    Article  CAS  Google Scholar 

  5. Claramunt I, Perez L. Estimation of measurement uncertainty in the determination of orthophosphates in seawater by continuous flow analysis (CFA). Accred Qual Assur, 2014,19(3):205–212

    Article  CAS  Google Scholar 

  6. Chen Y, He C, Cheng JJ, et al. Measurement uncertainty assessment of magnesium trisilicate column for determination of sudan colorants in food by HPLC using C8 column. J Huazhong Univ Sci Technolog Med Sci, 2016,36(5)752–757

    Article  PubMed  CAS  Google Scholar 

  7. Ramsey MH, Ellison SLR. Uncertainty factor: an alternative way to express measurement uncertainty in chemical measurement. Accred Qual Assur, 2015,20(2):153–155

    Article  Google Scholar 

  8. Ramser MH, Ellison SL. Ellison uncertainty factor: an alternative way to express measurement uncertainty in chemical measurement. Accred Qual Assur, 2015,20(2):153–155

    Article  Google Scholar 

  9. Yu ZR, Ning Y, YuH, et al. A HPLC-Q-TOFMS-based urinary metabolomic approach to identification of potential biomarkers of metabolic syndrome. J Huazhong Univ Sci Technolog Med Sci, 2014,34(2):276–283

    Article  PubMed  CAS  Google Scholar 

  10. Liu YN, Huang JG, Liu JM, et al. A simple and sensitive liquid chromatographic technique for the determination of cefotetan disodium in human plasma and its application in a pharmacokinetic study. J Huazhong Univ Sci Technolog Med Sci, 2012,32(5):779–784

    Article  PubMed  CAS  Google Scholar 

  11. Chen Y, Shao SW, Shen GX, et al. Determination of Fancy red in Puffing Food by HPLC. Zhong Guo Min Kang Yi Xue (Chinese), 2006,18(10):837–838

    Google Scholar 

  12. Kristiansen J. Description of a generally applicable model for the evaluation of uncertainty of measurement in clinical chemistry. Clin Chem Lab Med, 2005,39(10):920–931

    Google Scholar 

  13. Maroto A, Boque R, Rius FX. Estimating uncertainties of analytical results using information from the validation process. Analytica Chimica Acta, 1999, 391(99):173–185

    Article  CAS  Google Scholar 

  14. Ma ZD, Wang FC, Hao DS. The uncertainty evaluation of determination of aflatoxin by HPLC post-column derivation method. Acta Metrologica Sinica, 2002,23(3):229–240

    Google Scholar 

  15. Basak S, Kundu D. Evaluation of measurement uncertainty components associated with the results of complexometric determination of calcium in ceramic raw materials using EDTA. Accred Qual Assur, 2013,18(3):235–241

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jia-fa Liu.

Additional information

The paper is supported by the Foundation of Study on Early Damage and Markers of Atmospheric Particulates in Hubei province (No. WJ2015MB123).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y., Zhao, Y., Wang, HM. et al. Measurement Uncertainty Assessment for Determination of Allura Red in Puffed Food by HPLC. CURR MED SCI 38, 324–328 (2018). https://doi.org/10.1007/s11596-018-1882-5

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-018-1882-5

Key words

Navigation