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Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits

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Summary

This study was designed to evaluate the effects of drilling through the growth plate and using adipose-derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) to treat femoral head epiphyseal ischemic necrosis, which can be done in juvenile rabbits. Passagefour bromodeoxyuridine (BrdU)-labeled ADSCs were cultured, assayed with MTT to determine their viability and stained with alizarin red dye to determine their osteogenic ability. Two-month-old, healthy male rabbits (1.2 to 1.4 kg, n=45) underwent ischemic induction and were randomly divided into five groups (group A: animal model control; group B: drilling; group C: drilling & ADSCs; group D: drilling & BMP-2; and group E: drilling & ADSCs & BMP-2). Magnetic resonance imaging (MRI), X-ray imaging, hematoxylin and eosin staining and BrdU immunofluorescence detection were applied 4, 6 and 10 weeks after treatment. Approximately 90% of the ADSCs were labeled with BrdU and showed good viability and osteogenic ability. Similar results were observed in the rabbits in groups C and E at weeks 6 and 10. The animals of groups C and E demonstrated normal hip structure and improved femoral epiphyseal quotients and trabecular areas compared with those of the groups A and B (P<0.01). Group D demonstrated improved femoral epiphyseal quotients and trabecular areas compared with those of groups A and B (P<0.05). In summary, drilling through the growth plate combined with ADSC and BMP-2 treatments induced new bone formation and protected the femoral head epiphysis from collapsing in a juvenile rabbit model of femoral head epiphyseal ischemic necrosis.

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Acknowledgements

We thank the Third Xiangya Hospital of Central South University for supporting our research.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, Third Xiangya Hospital of Central South University, Changsha, 410013, China

    Zi-li Wang, Rong-zhen He, Jin-shen He, Xu Cao, Han-song Xia, Hong-liang Ba & Song Wu

  2. Department of Orthopaedics, Leping City People’s Hospital, Leping, 333399, China

    Bin Tu

  3. Department of Plastic Surgery, Third Xiangya Hospital of Central South University, Changsha, 410013, China

    Cheng Peng

  4. Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China

    Kun Xiong

Authors
  1. Zi-li Wang
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  2. Rong-zhen He
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  3. Bin Tu
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  4. Jin-shen He
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  5. Xu Cao
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  7. Hong-liang Ba
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  8. Song Wu
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  9. Cheng Peng
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  10. Kun Xiong
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Corresponding authors

Correspondence to Song Wu, Cheng Peng or Kun Xiong.

Additional information

This project was supported by the National Natural Science Foundation of China (No. 81572150, No. 81571939, No. 81301636 and No. 81772134), the Natural Science Foundation of Hunan Province (No. 13JJ2013 and No.2015JJ2187), and the Wu Jie-Ping Medical Foundation of the Minister of Health of China (No. 320.6750.14118).

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Wang, Zl., He, Rz., Tu, B. et al. Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits. CURR MED SCI 38, 277–288 (2018). https://doi.org/10.1007/s11596-018-1876-3

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  • DOI: https://doi.org/10.1007/s11596-018-1876-3

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