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Effects of meglumine cyclic adenylate pretreatment on systemic inflammatory response syndrome induced by lipopolysaccharide in rats

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Summary

Studies showed that the use of cyclic adenosine monophosphate (cAMP) substitutes or intracellular cAMP activators increased intracellular cAMP level, causing anti-inflammatory effects. This study was to investigate the effects of pretreatment with meglumine cyclic adenylate (MCA), a compound of meglumine and cAMP, on systemic inflammation induced by lipopolysaccharide (LPS) in rats. Eighteen adult male Sprague-Dawley rats were randomly divided into 3 groups (n=6 each): control group (NS group), LPS group (LPS group) and LPS with MCA pretreatment group (MCA group). Systemic inflammation was induced with LPS 10 mg/kg injected via the femoral vein in LPS and MCA groups. In MCA group, MCA 2 mg/kg was injected via the femoral vein 20 min before LPS injection, and the equal volume of normal saline was given in NS and LPS groups at the same time. Three hours after LPS injection, the blood samples were taken from the abdominal aorta for determination of plasma concentrations of TNF-α, IL-1, IL-6, IL-10, cAMP by ELISA and NF-κBp65 expression by Western blotting. The experimental results showed that inflammatory and antiinflammatory indices were increased in LPS group compared to NS group; inflammatory indices were declined and anti-inflammatory indices were increased in MCA group relative to LPS group. Our study suggested that MCA pretreatment may attenuate LPS-induced systemic inflammation.

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Authors and Affiliations

  1. Department of Anesthesiology, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Sciense and Technology, Wuhan, 430014, China

    Wei Liu  (刘 薇), Jing-li Chen  (陈璟莉) & Hong Yan  (严 虹)

  2. Department of Anesthesiology & Perioperative Medicine, Drexel University College of Medicine/Hahnemann University Hospital, Philadelphia, 19102, USA

    Henry Liu  (刘恒义)

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  1. Wei Liu  (刘 薇)
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  2. Jing-li Chen  (陈璟莉)
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  3. Henry Liu  (刘恒义)
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  4. Hong Yan  (严 虹)
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Correspondence to Hong Yan  (严 虹).

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Both authors contributed equally to this work.

This work was financially supported by the Science and Technology Bureau of Wuhan, Hubei Province, China (No. 2013060602010255).

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Liu, W., Chen, Jl., Liu, H. et al. Effects of meglumine cyclic adenylate pretreatment on systemic inflammatory response syndrome induced by lipopolysaccharide in rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 37, 332–336 (2017). https://doi.org/10.1007/s11596-017-1736-6

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  • DOI: https://doi.org/10.1007/s11596-017-1736-6

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