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Anti-inflammatory and analgesic effects of Bi-yuan-ling granules

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Summary

Bi-yuan-ling granule (BLG) is a traditional Chinese medicine compound composed mainly of baicalin and chlorogenic acid. It has been demonstrated to be clinically effective for various inflammatory diseases such as acute rhinitis, chronic rhinitis, atrophic rhinitis and allergic rhinitis. However, the underlying mechanisms of BLG against these diseases are not fully understood. This study aimed to explore the anti-inflammatory and analgesic activities of BLG, and examine its protective effects on mouse acute lung injury (ALI). The hot plate test and acetic acid-induced writhing assay in Kunming mice were adopted to evaluate the pain-relieving effects of BLG. The anti-inflammatory activities of BLG were determined by examining the effects of BLG on xylene-caused ear swelling in Kunming mice, the cotton pellet-induced granuloma in rats, carrageenan-induced hind paw edema and lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. The results showed that BLG at 15.5 mg/g could significantly relieve the pain by 82.5% (P<0.01) at 1 h after thermal stimulation and 91.2% (P<0.01) at 2 h after thermal stimulation. BLG at doses of 7.75 and 15.5 mg/g reduced the writhing count up to 33.3% (P<0.05) and 53.4% (P<0.01), respectively. Additionally, the xylene-induced edema in mice was markedly restrained by BLG at 7.75 mg/g (P<0.05) and 15.5 mg/g (P<0.01). BLG at 5.35 and 10.7 mg/g significantly reduced paw edema by 34.8% (P<0.05) and 37.9% (P<0.05) at 5 h after carrageenan injection. The granulomatous formation of the cotton pellet was profoundly suppressed by BLG at 2.68, 5.35 and 10.7 mg/g by 15.4%, 38.2% (P<0.01) and 58.9% (P<0.001), respectively. BLG also inhibited lung W/D ratio and the release of prostaglandin E2 (PGE2) in ALI mice. In addition, the median lethal dose (LD50), median effective dose (ED50) and half maximal inhibitory concentration (IC50) of BLG were found to be 42.7, 3.2 and 12.33 mg/g, respectively. All the findings suggest that BLG has significantly anti-inflammatory and analgesic effects and it may help reduce the damage of ALI.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, South-central University for Nationalities, Wuhan, 430074, China

    Xiao-bing Chen  (陈晓冰), Xian Yin  (尹 娴), Feng He  (何 峰), Yong-shen Ren  (任永申), Kang Dai  (戴 康) & Mei-xian Xiang  (向梅先)

  2. Department of Laboratory Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Han-wen Su  (苏汉文) & Huan-xiang Liu  (刘环香)

  3. Department of Pharmacology, The Pennsylvania State University College of Medicine and Milton S. Hershey Medical Center, Hershey, 17033, USA

    Mei-xian Xiang  (向梅先)

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  1. Xiao-bing Chen  (陈晓冰)
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  2. Han-wen Su  (苏汉文)
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  3. Huan-xiang Liu  (刘环香)
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  4. Xian Yin  (尹 娴)
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  5. Feng He  (何 峰)
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  6. Yong-shen Ren  (任永申)
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  7. Kang Dai  (戴 康)
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Correspondence to Mei-xian Xiang  (向梅先).

Additional information

This work was supported by the National Natural Science Foundation of China (No. 31200264), the Natural Science Foundation of Hubei Province (No. 2015BCA268), “Chenguang Planning” from Natural Science Foundation of Wuhan City (Nos. 2015070404010201 and 2014070404010210).

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Chen, Xb., Su, Hw., Liu, Hx. et al. Anti-inflammatory and analgesic effects of Bi-yuan-ling granules. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 456–462 (2016). https://doi.org/10.1007/s11596-016-1608-5

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  • DOI: https://doi.org/10.1007/s11596-016-1608-5

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