Skip to main content
SpringerLink
Log in

Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

Age-related hearing loss (AHL) is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1 (Kir5.1) plays a vital role in regulating cochlear K+ circulation which is necessary for normal hearing. The distribution of Kir5.1 in C57BL/6J mice cochleae, and the relationship between the expression of Kir5.1 and the etiology of AHL were investigated. Forty C57BL/6J mice were randomly divided into four groups at 4, 12, 24 and 52 weeks of age respectively. The location of Kir5.1 was detected by immunofluorescence technique. The mRNA and protein expression of Kir5.1 was evaluated in mice cochleae using real-time polymerase-chain reactions (RT-PCR) and Western blotting respectively. Kir5.1 was detected in the type II and IV fibrocytes of the spiral ligament in the cochlear lateral wall of C57BL/6J mice. The expression levels of Kir5.1 mRNA and protein in the cochleae of aging C57BL/6J mice were down-regulated. It was suggested that the age-related decreased expression of Kir5.1 in the lateral wall of C57BL/6J mice was associated with hearing loss. Our results indicated that Kir5.1 may play an important role in the pathogenesis of AHL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yamasoba T, Lin FR, Someya S, et al. Current concepts in age-related hearing loss: Epidemiology and mechanistic pathways. Hear Res, 2013, 303(9): 30–38

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Huang Q, Tang J. Age-related hearing loss or presbycusis. Eur Arch Otorhinolaryngol, 2010, 267(8): 1179–1191

    Article  PubMed  Google Scholar 

  3. Sepúlveda FV, Pablo Cid L, Teulon J, et al. Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels. Physiol Rev, 2015, 95(1): 179–217

    Article  PubMed  PubMed Central  Google Scholar 

  4. Tucker SJ, Imbrici P, Salvatore L, et al. pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia. J Biol Chem, 2000, 275(22): 16404–16407

    Article  CAS  PubMed  Google Scholar 

  5. Lourdel S, Paulais M, Cluzeaud F, et al. An inward rectifier K(+) channel at the basolateral membrane of the mouse distal convoluted tubule: similarities with Kir4-Kir5.1 heteromeric channels. J Physiol, 2002, 538(Pt 2): 391–404

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Derst C, Karschin C, Wischmeyer E, et al. Genetic and functional linkage of Kir5.1 and Kir2. 1 channel subunits. FEBS Lett, 2001, 491(3): 305–311

    Article  CAS  PubMed  Google Scholar 

  7. Hibino H, Higashi-Shingai K, Fujita A, et al. Expression of an inwardly rectifying K+ channel, Kir5.1, in specific types of fibrocytes in the cochlear lateral wall suggests its functional importance in the establishment of endocochlear potential. Eur J Neurosci, 2004, 19(1): 76–84

    Article  PubMed  Google Scholar 

  8. Hibino H, Kurachi Y. Molecular and physiological bases of the K+ circulation in the mammalian inner ear. Physiology (Bethesda), 2006, 21: 336–345

    Article  CAS  Google Scholar 

  9. Chen J, Zhao HB. The role of an inwardly rectifying K(+) channel (Kir4. 1) in the inner ear and hearing loss. Neuroscience, 2014, 265: 137–146

    CAS  PubMed  Google Scholar 

  10. Yang H, Xiong H, Huang Q, et al. Compromised potassium recycling in the cochlea contributes to conservation of endocochlear potential in a mouse model of age-related hearing loss. Neurosci Lett, 2013, 25, 555: 97–101

    Article  Google Scholar 

  11. Rozengurt N, Lopez I, Chiu CS, et al. Time course of inner ear degeneration and deafness in mice lacking the Kir4. 1 potassium channel subunit. Hear Res, 2003, 177(1-2): 71–80

    CAS  PubMed  Google Scholar 

  12. Hao X, Chu H, Zhou X, et al. Simultaneously reduced NKCC1 and Na, K-ATPase expression in murine cochlear lateral wall contribute to conservation of endocochlear potential following a sensorineural hearing loss. Neurosci Lett, 2011, 488(2): 204–209

    Article  Google Scholar 

  13. Wang Y, Chu H, Zhou L, et al. Correlation of PDCD5 and apoptosis in hair cells and spiral ganglion neurons of different age of C57BL/6J mice. J Huazhong Uni Sci Technol Med Sci, 2012, 32(1): 113–118

    Article  Google Scholar 

  14. Chen J, Chu H, Xiong H, et al. Downregulation of Cav1. 3 calcium channel expression in the cochlea is associated with age-related hearing loss in C57BL/6J mice. Neuroreport, 2013, 17, 24(6): 313–317

    Google Scholar 

  15. Park SN, Back SA, Park KH, et al. Comparison of cochlear morphology and apoptosis in mouse models of presbycusis. Clin Exp Otorhinolaryngol, 2010, 3(3): 126–135

    Article  PubMed  PubMed Central  Google Scholar 

  16. Gates GA, Mills JH. Presbycusis. The Lancet, 2005, 366(9491): 1111–1120

    Article  Google Scholar 

  17. Takeuchi S, Ando M, Sato T, et al. Three-dimensional and ultrastructural relationships between intermediate cells and capillaries in the gerbil stria vascularis. Hear Res, 2001, 155(1-2): 103–112

    Article  CAS  PubMed  Google Scholar 

  18. Hao X, Xing Y, Moore MW, et al. Sox10 expressing cells in the lateral wall of the aged mouse and human cochlea. Plos One, 2014, 9(6): e97389

    Article  PubMed  PubMed Central  Google Scholar 

  19. Schulte BA, Steel KP. Expression of α and β subunit isoforms of Na, K-ATPase in the mouse inner ear and changes with mutations at the W v or Sl d loci. Hear Res, 1994, 78: 65–76

    Article  CAS  PubMed  Google Scholar 

  20. Liu Y, Chu H, Chen J, et al. Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice. Acta Otolaryngol, 2014, 134(10): 1047–1051

    Article  CAS  PubMed  Google Scholar 

  21. Fumiaki N, Hiroshi H, Katsumi D, et al. The endocochlear potential depends on two K+ diffusion potentials and an electrical barrier in the stria vascularis of the inner ear. Proc Natl Acad Sci USA, 2008, 105(5): 1751–1756

    Article  Google Scholar 

  22. Shinichi S, Jinze X, Kenji K, et al. Age-related hearing loss in C57BL/6J mice is mediated by Bak-dependent mitochondrial apoptosis. Proc Natl Acad Sci USA, 2009, 106(46): 19432–19437

    Article  Google Scholar 

  23. Ichimiya I, Suzuki M, Mogi G. Age-related changes in the murine cochlear lateral wall. Hear Res, 2000, 139(1-2): 116–122

    Article  CAS  PubMed  Google Scholar 

  24. Ohlemiller KK, Lett JM, Gagnon PM. Cellular correlates of age-related endocochlear potential reduction in a mouse model. Hear Res, 2006, 220(1): 10–26

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Chun-chen Pan  (潘春晨), Han-qi Chu  (褚汉启), Yan-bing Lai  (赖彦冰), Yan-bo Sun  (孙彦博), Zhi-hui Du  (杜智会), Yun Liu  (刘 云), Jin Chen  (陈 金), Ting Tong  (童 婷), Qing-guo Chen  (陈请国), Liang-qiang Zhou  (周良强), Dan Bing  (冰 丹) & Yan-ling Tao  (陶雁玲)

Authors
  1. Chun-chen Pan  (潘春晨)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Han-qi Chu  (褚汉启)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan-bing Lai  (赖彦冰)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan-bo Sun  (孙彦博)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhi-hui Du  (杜智会)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yun Liu  (刘 云)
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jin Chen  (陈 金)
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ting Tong  (童 婷)
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Qing-guo Chen  (陈请国)
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Liang-qiang Zhou  (周良强)
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Dan Bing  (冰 丹)
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Yan-ling Tao  (陶雁玲)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yan-ling Tao  (陶雁玲).

Additional information

This project was supported by the National Natural Science Foundation of China (Nos. 81271078, 81300827, and 81500791).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pan, Cc., Chu, Hq., Lai, Yb. et al. Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 406–409 (2016). https://doi.org/10.1007/s11596-016-1600-0

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-016-1600-0

Key words

Search

Navigation