Total triterpenoids from Ganoderma Lucidum suppresses prostate cancer cell growth by inducing growth arrest and apoptosis

Summary

In this study, one immortalized human normal prostatic epithelial cell line (BPH) and four human prostate cancer cell lines (LNCaP, 22Rv1, PC-3, and DU-145) were treated with Ganoderma Lucidum triterpenoids (GLT) at different doses and for different time periods. Cell viability, apoptosis, and cell cycle were analyzed using flow cytometry and chemical assays. Gene expression and binding to DNA were assessed using real-time PCR and Western blotting. It was found that GLT dose-dependently inhibited prostate cancer cell growth through induction of apoptosis and cell cycle arrest at G1 phase. GLT-induced apoptosis was due to activation of Caspases-9 and -3 and turning on the downstream apoptotic events. GLT-induced cell cycle arrest (mainly G1 arrest) was due to up-regulation of p21 expression at the early time and down-regulation of cyclin-dependent kinase 4 (CDK4) and E2F1 expression at the late time. These findings demonstrate that GLT suppresses prostate cancer cell growth by inducing growth arrest and apoptosis, which might suggest that GLT or Ganoderma Lucidum could be used as a potential therapeutic drug for prostate cancer.

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Correspondence to An-yang Wei 韦安阳 or Jin-ming Di 狄金明 or Heng-jun Xiao 肖恒军 or Zhi-gang Zhang 张志刚 or Liu-hong Cai 蔡柳洪 or Xin Tao 陶 欣 or Tao Qi 齐 涛 or Di-ling Chen 陈地灵 or Jun Chen 陈 俊.

Additional information

The authors contributed equally to this work.

This project was supported by Science and Technology Planning Project of Guangdong Province, China (No. 2011B080701075).

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Wang, T., Xie, Z., Huang, Z. et al. Total triterpenoids from Ganoderma Lucidum suppresses prostate cancer cell growth by inducing growth arrest and apoptosis. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 35, 736–741 (2015). https://doi.org/10.1007/s11596-015-1499-x

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Key words

  • Ganoderma Lucidum triterpenoids
  • prostate cancer
  • cell cycle arrest
  • apoptosis