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Antioxidant and antigenotoxic activity of bioactive extracts from corn tassel

Journal of Huazhong University of Science and Technology [Medical Sciences] volume 34, pages 131–136 (2014)Cite this article

Summary

This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts (CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test (strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner (P<0.01). It is concluded that these integrated approaches to antioxidant and antigenotoxicity assessment may be useful to study corn tassel as a natural herbal material.

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Affiliations

  1. Institute of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

    Li-chun Wang, Ya-qin Yu, Min Fang, Cai-gui Zhan, Hong-yan Pan, Yong-ning Wu & Zhi-yong Gong

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  1. Li-chun Wang
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  2. Ya-qin Yu
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  3. Min Fang
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  4. Cai-gui Zhan
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  5. Hong-yan Pan
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  6. Yong-ning Wu
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  7. Zhi-yong Gong
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Correspondence to Zhi-yong Gong.

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Both authors contributed equally to this work.

This project was supported by the National Natural Science Foundation of China (No. 21077082).

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Wang, Lc., Yu, Yq., Fang, M. et al. Antioxidant and antigenotoxic activity of bioactive extracts from corn tassel. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 34, 131–136 (2014). https://doi.org/10.1007/s11596-014-1244-x

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  • DOI: https://doi.org/10.1007/s11596-014-1244-x

Key words

  • corn tassel
  • antioxidant activity
  • antigenotoxicity activity
  • extraction
  • Ames test
  • 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide