Role of GSK-3β in isoflurane-induced neuroinflammation and cognitive dysfunction in aged rats

  • Shi-yong Li (李世勇)
  • Xin Chen (陈 欣)
  • Ye-ling Chen (陈晔凌)
  • Lei Tan (谭 蕾)
  • Yi-lin Zhao (赵以林)
  • Jin-tao Wang (王金韬)
  • Qiang Xiang (向 强)
  • Ai-lin Luo (罗爱林)
Article

Summary

This study investigated the role of glycogen synthase kinase-3β (GSK-3β) in isoflurane-induced neuroinflammation and cognitive dysfunction in aged rats. The hippocampi were dissected from aged rats which had been intraperitoneally administered lithium chloride (LiCl, 100 mg/kg) and then exposed to 1.4% isoflurane for 6 h. The expression of GSK-3β was detected by Western blotting. The mRNA and protein expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were measured by real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Morris water maze was employed to detect spatial memory ability of rats. The results revealed that the level of GSK-3β was upregulated after isofurane exposure. Real-time PCR analysis demonstrated that isoflurane anesthesia increased mRNA levels of TNF-α, IL-1β and IL-6, which was consistent with the ELISA results. However, these changes were reversed by prophylactic LiCl, a non-selective inhibitor of GSK-3β. Additionally, we discovered that LiCl alleviated isoflurane-induced cognitive impairment in aged rats. Furthermore, the role of GSK-3β in isoflurae-induced neuroinflammation and cognitive dysfunction was associated with acetylation of NF-κB p65 (Lys310). In conclusion, these results suggested that GSK-3β is associated with isoflurane-induced upregulation of proinflammatory cytokines and cognitive disorder in aged rats.

Key words

isoflurane GSK-3β cognitive dysfunction neuroinflammation 

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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Shi-yong Li (李世勇)
    • 1
  • Xin Chen (陈 欣)
    • 1
  • Ye-ling Chen (陈晔凌)
    • 1
  • Lei Tan (谭 蕾)
    • 1
  • Yi-lin Zhao (赵以林)
    • 1
  • Jin-tao Wang (王金韬)
    • 1
  • Qiang Xiang (向 强)
    • 2
  • Ai-lin Luo (罗爱林)
    • 1
  1. 1.Department of Anesthesiology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Anesthesiology, Wuhan Children’s Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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