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Regulatory effects of deguelin on proliferation and cell cycle of Raji cells

Journal of Huazhong University of Science and Technology [Medical Sciences] volume 33, pages 491–495 (2013)Cite this article

Summary

The underlying mechanism of deguelin regulating the cell cycle in human Burkitt’s lymphoma cell line Raji cells in vitro, and the cytotoxicity of deguelin to Raji cells and human peripheral blood monocular cells (PBMCs) were investigated. The effects of deguelin on the growth of Raji cells were studied by 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) assay. Apoptosis was detected through Hoechst 33258 staining. The effect of deguelin on the cell cycle of Raji cells was studied by a propidium iodide method. The expression levels of cyclin D1, P21 and pRb were examined by using Western blotting. The results showed that the proliferation of Raji cells was inhibited in the deguelin-treated group, with a 24-h IC50 value of 21.61 nmol/L and a 36-h IC50 value of 17.07 nmol/L. Proliferation in Raji cells was inhibited significantly by deguelin, while little change was observed in PBMCs. Deguelin induced G2/M arrest in Raji cells. The expression of cyclin D1, P21 and pRb was dramatically down-regulated by deguelin in a dose-dependent manner. It was concluded that deguelin could inhibit the proliferation of Raji cells by arresting the cells at G2/M phase and inducing the cell apoptosis. Moreover, deguelin selectively induced apoptosis of Raji cells with low toxicity to PBMCs. The antitumor effects of deguelin were related to the down-regulated expression of cyclin D1, P21 and pRb proteins.

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Affiliations

  1. No. 457th Hospital of PLA, Wuhan, 430012, China

    Jin-rong Xiong

  2. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Hong-li Liu

Authors
  1. Jin-rong Xiong
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  2. Hong-li Liu
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Correspondence to Hong-li Liu.

Additional information

This project was supported by grants from the National Natural Science Foundation of China (No. 30900597) and the Science Foundation of Hubei Health Department (No. JX6B08).

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Xiong, Jr., Liu, Hl. Regulatory effects of deguelin on proliferation and cell cycle of Raji cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 491–495 (2013). https://doi.org/10.1007/s11596-013-1147-2

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  • DOI: https://doi.org/10.1007/s11596-013-1147-2

Key words

  • deguelin
  • cell cycle
  • apoptosis
  • Burkitt’s lymphoma