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Salidroside attenuates LPS-stimulated activation of THP-1 cell-derived macrophages through down-regulation of MAPK/NF-kB signaling pathways

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Summary

Excessive activation of macrophages is implicated in various inflammatory injuries. Salidroside (Sal), one of the main bioactive components of Rhodiola Sachalinensis, has been reported to possess anti-inflammatory activities. This study aimed to examine the effect of Sal on the activation of macrophages and the possible mechanism. The lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were established. The changes in the inflammatory profiles of THP-1-derived macrophages were determined. The results showed that Sal significantly decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), interleukin-1beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) at both mRNA and protein levels in THP-1-derived macrophages, and the effect was dose-depedent. Moreover, NF-κB activation was significantly suppressed and the phosphorylation of ERK, p38 and JNK was substantially down-regulated after Sal treatment. The findings suggested that Sal can suppress the activation of LPS-stimulated PMA-differetiated THP-1 cells, as evidenced by the decreased expression of iNOS, COX2, IL-1β, IL-6 and TNF-α, and the mechanism involves the inhibition of NF-κB activation and the phosphorylation of the MAPK signal pathway.

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Authors and Affiliations

  1. Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Hong-wu Wang  (王洪武), Ting Wu  (吴 婷), Jun-ying Qi  (齐俊英), Ya-qi Wang  (王亚琦) & Qin Ning  (宁 琴)

  2. Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xiao-ping Luo  (罗小平)

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  1. Hong-wu Wang  (王洪武)
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  2. Ting Wu  (吴 婷)
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  3. Jun-ying Qi  (齐俊英)
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Correspondence to Qin Ning  (宁 琴).

Additional information

This project was supported by grants from the National Natural Science Foundation of China (Nos. 81100282, 81030007, 81171558, 81271808), Program for Changjiang Scholars and Innovative Research Team in University (No. PCSIRT1131), and China Postdoctoral Science Foundation (No. 2013M531700).

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Wang, Hw., Wu, T., Qi, Jy. et al. Salidroside attenuates LPS-stimulated activation of THP-1 cell-derived macrophages through down-regulation of MAPK/NF-kB signaling pathways. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 463–469 (2013). https://doi.org/10.1007/s11596-013-1143-6

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  • DOI: https://doi.org/10.1007/s11596-013-1143-6

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