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Exercise training attenuated chronic cigarette smoking-induced up-regulation of FIZZ1/RELMα in lung of rats

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Summary

FIZZ/RELM is a new gene family named “found in inflammatory zone” (FIZZ) or “resistin-like molecule” (RELM). FIZZ1/RELMα is specifically expressed in lung tissue and associated with pulmonary inflammation. Chronic cigarette smoking up-regulates FIZZ1/RELMα expression in rat lung tissues, the mechanism of which is related to cigarette smoking-induced airway hyperresponsiveness. To investigate the effect of exercise training on chronic cigarette smoking-induced airway hyperresponsiveness and up-regulation of FIZZ1/RELMα, rat chronic cigarette smoking model was established. The rats were treated with regular exercise training and their airway responsiveness was measured. Hematoxylin and eosin (HE) staining, immunohistochemistry and in situ hybridization of lung tissues were performed to detect the expression of FIZZ1/RELMα. Results revealed that proper exercise training decreased airway hyperresponsiveness and pulmonary inflammation in rat chronic cigarette smoking model. Cigarette smoking increased the mRNA and protein levels of FIZZ1/RELMα, which were reversed by the proper exercise. It is concluded that proper exercise training prevents up-regulation of FIZZ1/RELMα induced by cigarette smoking, which may be involved in the mechanism of proper exercise training modulating airway hyperresponsiveness.

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References

  1. Holcomb IN, Kabakoff RC, Chan B, et al. FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family. EMBO J, 2000,19(15):4046–4055

    Article  PubMed  CAS  Google Scholar 

  2. Steppan CM, Brown EJ, Wright CM, et al. A family of tissue-specific resistin-like molecules. Proc Natl Acad Sci USA, 2001,98(2):502–506

    Article  PubMed  CAS  Google Scholar 

  3. Yang RZ, Huang Q, Xu A, et al. Comparative studies of resistin expression and phylogenomics in human and mouse. Biochem Biophys Res Commun, 2003,310(3): 927–935

    Article  PubMed  CAS  Google Scholar 

  4. Gerstmayer B, Küsters D, Gebel S, et al. Identification of RELMgamma, a novel resistin-like molecule with a distinct expression pattern. Genomics, 2003,81(6):588–595

    Article  PubMed  CAS  Google Scholar 

  5. Bełtowski J. Adiponectin and resistin—new hormones of white adipose tissue. Med Sci Monit, 2003,9(2): RA55–RA61

    PubMed  Google Scholar 

  6. Teng X, Li D, Champion HC, et al. FIZZ1/RELMalpha, a novel hypoxia-induced mitogenic factor in lung with vasoconstrictive and angiogenic properties. Circ Res, 2003,92(10):1065–1067

    Article  PubMed  CAS  Google Scholar 

  7. Ye H, Ma W, Zhang S, et al. Chronic cigarette smoking induces alteration of FIZZ1/RELMα expression in rat lung. Chin J Pathophysiol (Chinese), 2006,22(5):938–942

    CAS  Google Scholar 

  8. Garcia-Aymerich J, Lange P, Benet M, et al. Regular physical activity modifies smoking-related lung function decline and reduces risk of chronic obstructive pulmonary disease: a population-based cohort study. Am J Respir Crit Care Med, 2007,175(5):458–463

    Article  PubMed  Google Scholar 

  9. Bonsignore MR, Scichilone N, Morici G. Bronchial responsiveness and airway inflammation in trained subjects. Thorax, 2008,63(1):90–91

    Article  PubMed  CAS  Google Scholar 

  10. Ye H, Du L, Wu H, et al. Effect of exercise stress on cigarette smoke-induced downregulation of BKca and Kv1.5 expression in rat bronchial smooth muscle cells. Chin J Pathophysiol (Chinese), 2004,20(11):1999–2004

    CAS  Google Scholar 

  11. Ohga K, Kuromitsu S, Takezawa R, et al. YM-341619 suppresses the differentiation of spleen T cells into Th2 cells in vitro, eosinophilia, and airway hyperresponsiveness in rat allergic models. Eur J Pharmacol, 2008,590(1–3):409–416

    Article  PubMed  CAS  Google Scholar 

  12. Matsubara S, Fushimi K, Ogawa K, et al. Inhibition of pulmonary eosinophilia does not necessarily prevent the airway hyperresponsiveness induced by Sephadex beads. Int Arch Allergy Immunol, 1998,116(1):67–75

    Article  PubMed  CAS  Google Scholar 

  13. Taito S, Sekikawa K, Domen S, et al. Pulmonary oxidative stress is induced by maximal exercise in young cigarette smokers. Nicotine Tob Res, 2012,14(2):243–247

    Article  PubMed  CAS  Google Scholar 

  14. Shaaban R, Leynaert B, Soussan D, et al. Physical activity and bronchial hyperresponsiveness: European Community Respiratory Health Survey II. Thorax, 2007,62(5): 403–410

    Article  PubMed  Google Scholar 

  15. Scichilone N, Morici G, Marchese R, et al. Reduced airway responsiveness in nonelite runners. Med Sci Sports Exerc, 2005,37(12):2019–2025

    Article  PubMed  Google Scholar 

  16. Bonsignore MR, Morici G, Riccobono L, et al. Airway inflammation in nonasthmatic amateur runners. Am J Physiol Lung Cell Mol Physiol, 2001,281(3):L668–L676

    PubMed  CAS  Google Scholar 

  17. Pastva A, Estell K, Schoeb TR, et al. Aerobic exercise attenuates airway inflammatory responses in a mouse model of atopic asthma. J Immunol, 2004,172(7): 4520–4526

    PubMed  CAS  Google Scholar 

  18. Chao PW, Ramsdell J, Renvall M, et al. Does a history of exercise in COPD patients affect functional status? A study using a lifetime physical activity questionnaire investigates a correlation between exercise and functional status as evidenced by six-minute walk distance. COPD, 2011,(6):429–436

    Article  Google Scholar 

  19. Ye H, Wu HH, Du L, et al. Effect of exercise stress on cigarette smoking-induced downregulation of BKca and Kv1.5 expression in pulmonary arterial smooth muscle cells of rats. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi (Chinese), 2006,24(4):218–221

    CAS  Google Scholar 

  20. Raes G, De Baetselier P, Noël W, et al. Differential expression of FIZZ1 and Ym1 in alternatively versus classically activated macrophages. J Leukoc Biol, 2002,71(4):597–602

    PubMed  CAS  Google Scholar 

  21. Yamaji-Kegan K, Su Q, Angelini DJ, et al. Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2. Am J Physiol Lung Cell Mol Physiol, 2006,291(8):L1159–L1168

    Article  PubMed  CAS  Google Scholar 

  22. Tong Q, Zheng L, Lin L, et al. Hypoxia-induced mitogenic factor promotes vascular adhesion molecule-1 expression via the PI-3K/Akt-NF-kappaB signaling pathway. Am J Respir Cell Mol Biol, 2006(4),35:444–456

    Article  Google Scholar 

  23. Hallberg L, Janelidze S, Engstrom G, et al. Exercise-induced release of cytokines in patients with major depressive disorder. J Affect Disord, 2010,126(1–2): 262–267

    Article  PubMed  CAS  Google Scholar 

  24. Silva RA, Vieira RP, Duarte AC, et al. Aerobic training reverses airway inflammation and remodeling in an asthma murine model. Eur Respir J, 2010,35(5):994–1002

    Article  PubMed  CAS  Google Scholar 

  25. Stütz AM, Pickart LA, Trifilieff A, et al. The Th2 cell cytokines IL-4 and IL-13 regulate found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein-dependent mechanism. J Immunol, 2003,170(4):1789–1796

    PubMed  Google Scholar 

  26. Liu T, Jin H, Ullenbruch M, et al. Regulation of found in inflammatory zone 1 expression in bleomycin-induced lung fibrosis: role of IL-4/IL-13 and mediation via STAT-6. J Immunol, 2004,173(5):3425–3431

    PubMed  CAS  Google Scholar 

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Correspondence to Hong Ye  (叶 红).

Additional information

This project was supported by grants from the National Natural Science Foundation of China (No. 81200020, 30770943, 30770648, and 31271490).

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Ma, Wl., Cai, Pc., Xiong, Xz. et al. Exercise training attenuated chronic cigarette smoking-induced up-regulation of FIZZ1/RELMα in lung of rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 22–26 (2013). https://doi.org/10.1007/s11596-013-1065-3

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  • DOI: https://doi.org/10.1007/s11596-013-1065-3

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