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Effect of p120 catenin silencing on biological behaviors of PANC-1 cells

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Summary

This study examined the possible role of p120ctn in the pathogenesis and development of pancreatic cancer. PANC-1 cells, a kind of human pancreatic carcinoma cell line, were cultured in this study. p120ctn was immunocytochemically detected in PANC-1 cells. The recombinant lentivirus vector was constructed to knock down the p120ctn expression of PANC-1 cells. Real-time quantitative PCR (RQ-PCR) and Western blotting were used to determine the expression of p120ctn and E-cadherin in PANC-1 cells after p120ctn knockdown. The adhesion, invasion and migration capacity of PANC-1 cells after p120ctn knockdown was detected by cell adhesion, invasion and migration assays. Cell growth was measured by the MTT method. Cell cycle and apoptosis were analyzed by fluorescence-activated cell sorting. The results showed that p120ctn knockdown led to significantly down-regulated E-cadherin and a reduced cell-to-cell adhesion ability in PANC-1 cells. shRNA-mediated knockdown of p120ctn reduced invasion and migration capacity of PANC-1 cells, inhibited cell growth, caused a significant decrease in the percentage of cells in G1, an increase in S, and promoted apoptosis of PANC-1 cells. It was concluded that p120ctn plays a pivotal role in the proliferation and metastasis of pancreatic carcinoma, suggesting that p120ctn is a novel target for pancreatic carcinoma treatment.

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Authors and Affiliations

  1. Department of General Surgery, the Affiliated Zhongda Hospital, Southeast University, Nanjing, 210009, China

    Zhangjun Cheng  (程张军), Xin Shi  (石 欣), Shibo Lin  (林士波) & Jiangyan Xia  (夏江燕)

  2. Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai, 200438, China

    Zhangjun Cheng  (程张军), Pinghua Yang  (杨平华) & Feng Shen  (沈 锋)

  3. Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, D-81675, Germany

    Zhangjun Cheng  (程张军), Volker Assfag & Norbert Hüser

Authors
  1. Zhangjun Cheng  (程张军)
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  2. Volker Assfag
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  3. Xin Shi  (石 欣)
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  4. Shibo Lin  (林士波)
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  5. Jiangyan Xia  (夏江燕)
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  6. Pinghua Yang  (杨平华)
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  7. Norbert Hüser
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  8. Feng Shen  (沈 锋)
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Corresponding author

Correspondence to Feng Shen  (沈 锋).

Additional information

This work was supported by the National Natural Science Foundation of China (No. 30700801).

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Cheng, Z., Assfag, V., Shi, X. et al. Effect of p120 catenin silencing on biological behaviors of PANC-1 cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 707–712 (2012). https://doi.org/10.1007/s11596-012-1022-6

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  • DOI: https://doi.org/10.1007/s11596-012-1022-6

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