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Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24

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Summary

Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative. This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad. The infiltrating bladder cancer cells T24 were cultured, and then treated by a proper dosage of drug. Their viability was a determined by MTT method. Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels. Moreover, immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad. The result showed that, at both mRNA and protein levels, the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group, while the β-Cat expression was also relocated from the cell nucleus to cytoplasm. Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.

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Authors and Affiliations

  1. School of Basic Medical Science, Wuhan University, Wuhan, 430071, China

    Eyou Wang  (王鄂友), Jun Li  (黎 俊), Guohua Yang  (杨国华) & Shan Zhong  (钟 山)

  2. Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Eyou Wang  (王鄂友)

  3. Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Tongzu Liu  (刘同族)

Authors
  1. Eyou Wang  (王鄂友)
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  2. Jun Li  (黎 俊)
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  3. Guohua Yang  (杨国华)
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  4. Shan Zhong  (钟 山)
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  5. Tongzu Liu  (刘同族)
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Corresponding authors

Correspondence to Shan Zhong  (钟 山) or Tongzu Liu  (刘同族).

Additional information

The two authors contributed equally to this work.

This project was supported by a grant from the National Natural Science Foundation of China (No. 30972975).

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Wang, E., Li, J., Yang, G. et al. Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 237–241 (2012). https://doi.org/10.1007/s11596-012-0042-6

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  • DOI: https://doi.org/10.1007/s11596-012-0042-6

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