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Effects of testosterone on norepinephrine release in isolated rat heart

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The effects of testosterone on norepinephrine release were investigated in the isolated rat hearts. Sprague-Dawley male rats (n=120) were randomized to testosterone and control groups. The rats in testosterone group were perfused with modified Krebs-Henseleit buffer containing different concentrations of testosterone (0.1, 1.0, 10.0, and 100.0 nmol/L, respectively). Myocardial ischemia was induced by globally stopping the perfusion flow. Exocytotic norepinephrine release was induced by electrical field stimulation at 5 V (effective voltage) and 6 Hz (pulse width of 2 ms) for 1 min. The overflow of norepinephrine was determined by high pressure liquid chromatography and electrochemical detection (HPLC-EC). Following acute ischemia, testosterone (1.0, 10.0 and 100.0 nmol/L) significantly reduced norepinephrine release (P<0.01), and the norepinepherine overflow was similar between the control and 0.1 nmol/L testosterone group (P>0.05). Electrical stimulation of the ventricle evoked norepinepherine release, and this was diminished by the perfusion with testosterone at the concentrations of 1.0, 10.0 and 100.0 nmol/L (P<0.01). It is suggested that testosterone suppresses ischemia- and electrical stimulation-induced norepinepherine release in the isolated rat hearts.

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Authors and Affiliations

  1. Department of Cardiology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China

    Xiaofei Wang  (王晓菲), Jun Bu  (卜 军), Linghong Shen  (沈玲红) & Ben He  (何 奔)

  2. Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China

    Yanzhou Zhang  (张彦周)

Authors
  1. Xiaofei Wang  (王晓菲)
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  2. Yanzhou Zhang  (张彦周)
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  3. Jun Bu  (卜 军)
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  4. Linghong Shen  (沈玲红)
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  5. Ben He  (何 奔)
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Corresponding author

Correspondence to Ben He  (何 奔).

Additional information

These authors contributed equally to this work.

This project was supported by grants from the National Natural Science Foundation of China (No. 30670880), and the Shanghai Municipal Natural Science Foundation (No. XD1402600).

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Wang, X., Zhang, Y., Bu, J. et al. Effects of testosterone on norepinephrine release in isolated rat heart. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 42–46 (2012). https://doi.org/10.1007/s11596-012-0007-9

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  • DOI: https://doi.org/10.1007/s11596-012-0007-9

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