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Combined pluronic P85- and ultrasound contrast agents-mediated gene transfection to HepG2 cells

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Summary

This study examined the effect of P85 (a pluronic block copolymer) and microbubble (MB) ultrasound contrast agents under ultrasound irradiation on gene transfection and expression. The pEGFP plasmids that can encode enhanced green fluorescent protein (pEGFP) served as a report gene and were mixed with different concentrations of MB/0.05% (w/v) P85. Then the plasmids were transfected into human hepatoma G2 (HepG2) cells. The HepG2 cells treated with MB/P85 or without treatment were exposed to ultrasound (US parameters: 1 MHz, 1.0 W/cm2, 20 s, 20% duty cycle). Twenty-four hours later, the transfection efficiency was assessed by fluorescence microscopy and fluorescence activated cell sorting (FACS) analysis. The cell viability was evaluated by Trypan blue exclusion test. The results showed that the gene transfection efficiency in HepG2 cells under ultrasound irradiation was significantly higher than that without ultrasound irradiation. HepG2 cells in the MB or P85 group in the absence of ultrasound expressed less amount of green fluorescent protein. The expression efficiency reached (22.14±3.06)% and the survival rate was as high as (55.73±3.32)% in the 30% MB plus P85 group. It was concluded that MB and P85 in the presence of ultrasound can enhance gene transfection and expression.

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References

  1. Kabanov A, Zhu J, Alkhov V. Pluronic block copolymers for gene delivery. Adv Genet, 2005, 53PA:231–261

    Article  PubMed  Google Scholar 

  2. Yang Z, Sahay G, Sriadibhatla S, et al. Amphiphilic block copolymers enhance cellular uptake and nuclear entry of polyplex-delivered DNA. Bioconjug Chem, 2008,19(10):1987–1994

    Article  PubMed  CAS  Google Scholar 

  3. Chen YC, Zhang QP, Liang HD, et al. Pluronic block copolymers: a novel function in ultrasound-mediated gene transfer. Chin J Ultrasonogr (Chinese), 2005,14(11):858–862

    Google Scholar 

  4. Krupka TM, Weinberg BD, Wu H, et al. Effect of intratumoral injection of carboplatin combined with pluronic P85 or L61 on experimental colorectal carcinoma in rats. Exp Biol Med (Maywood), 2007, 232(7):950–957

    CAS  Google Scholar 

  5. Yang Z, Zhu J, Sriadibhatla S, et al. Promoter-and strain-selective enhancement of gene expression in a mouse skeletal muscle by a polymer excipient pluronic P85. J Control Release, 2005,108(2–3):496–512

    Article  PubMed  CAS  Google Scholar 

  6. Batrakova EV, Li S, Li Y, et al. Effect of pluronic P85 on ATPase activity of drug efflux transporters. Pharm Res, 2004,21(12):2226–2233

    Article  PubMed  CAS  Google Scholar 

  7. Ran HT, Ren H, Wang ZG, et al. Experimental study of enhanced gene delivery to cultured tumor cells in vitro by ultrasound and contrast agent. Chin J Imaging Technol (Chinese), 2005,21(8):1151–1154

    Google Scholar 

  8. Zhang SJ, Li FH, Du J. Liposome enhanced transfection of pEGFP-N1 plasmid to MCF-7 in vitro with SonoVue combined with ultrasound. Chin J Imaging Technol (Chinese), 2009,25(8):1343–1346

    Google Scholar 

  9. Miller DL, Song J. Tumor growth reduction and DNA transfer by cavitation-enhanced high-intensity focused ultrasound in vivo. Ultrasound Med Biol, 2003,29(6):887–893

    Article  PubMed  Google Scholar 

  10. Batrakova EV, Li S, Alakhov VY, et al. Optimal structure requirements for pluronic block copolymers in modifying pglycoprotein drug efflux transporter activity in bovine brain microvessel endothelial cells. Pharmacol Exp Ther, 2003,304(2):845–854

    Article  CAS  Google Scholar 

  11. Gaurav S, Vivek G, Robert L, et al. The utilization of pathogen-like cellular trafficking by single chain block copolymer. Biomaterials, 2010,31(7):1757–1764

    Article  Google Scholar 

  12. Maskarinec SA, Hannig J, Lee RC, et al. Direct observation of poloxamer 188 insertion into lipid monolayers. Biophys, 2002,82(3):1453–1459

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xijun Zhang  (张喜君), Kaiyan Li  (李开艳), Xian Cui  (崔 贤), Liangjun Hu  (胡良军) & Yunchao Chen  (陈云超)

  2. Department of Ultrasound, Henan Provincial People’s Hospital, Zhengzhou, 450000, China

    Xijun Zhang  (张喜君)

Authors
  1. Xijun Zhang  (张喜君)
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  2. Kaiyan Li  (李开艳)
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  3. Xian Cui  (崔 贤)
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  4. Liangjun Hu  (胡良军)
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  5. Yunchao Chen  (陈云超)
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Corresponding author

Correspondence to Kaiyan Li  (李开艳).

Additional information

This project was supported by grants from National Natural Sciences Foundation of China (No. 30970882) and Natural Sciences Foundation of Hubei Province, China (No. 2008CDB-148).

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Zhang, X., Li, K., Cui, X. et al. Combined pluronic P85- and ultrasound contrast agents-mediated gene transfection to HepG2 cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 842–845 (2011). https://doi.org/10.1007/s11596-011-0688-5

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  • DOI: https://doi.org/10.1007/s11596-011-0688-5

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