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Triptolide-induced apoptosis by inactivating nuclear factor-kappa B apoptotic pathway in multiple myeloma in vitro

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Summary

The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro, as well as the roles of nuclear factor-kappa B (NF-κB) and IκBα was investigated. The effect of tritptolide on the growth of RPMI-8226 cells was studied by MTT assay. Apoptosis was detected by Hoechest 33258 staining and Annexin V/PI double staining assay. The expression of NF-κB and IκBα was observed by Western blot and confocal microscopy. The results showed that triptolide inactivated NF-κB apoptotic pathway in human multiple myeloma RPMI-8226 cells. Triptolide at nM range induced proliferation inhibition in a dose- and time-dependent manner and apoptosis in a dose-dependent fashion in RPMI-8226 cells. Besides, we observed the inhibition of NF-κB /p65 in the nuclear fraction was correlated with the increase in the protein expression of IκBα in the cytosol. These results suggested that triptolide might exhibit its strong anti-tumor effects via inactivation of NF-κB/p65 and IκBα.

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Authors and Affiliations

  1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Rong Zeng  (曾 蓉), Linlan Zeng  (曾令兰), Yan Chen  (陈 燕), Fei Zhao  (赵 菲), Rui Li  (李 睿), Lu Wen  (文 璐) & Chun Zhang  (张 纯)

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  1. Rong Zeng  (曾 蓉)
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  2. Linlan Zeng  (曾令兰)
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  3. Yan Chen  (陈 燕)
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  4. Fei Zhao  (赵 菲)
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  5. Rui Li  (李 睿)
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  6. Lu Wen  (文 璐)
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  7. Chun Zhang  (张 纯)
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Corresponding author

Correspondence to Chun Zhang  (张 纯).

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These author contributed equally to this work.

This project was supported by a grant from National Natural Sciences Foundation of China (No. 30700882).

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Zeng, R., Zeng, L., Chen, Y. et al. Triptolide-induced apoptosis by inactivating nuclear factor-kappa B apoptotic pathway in multiple myeloma in vitro . J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 446–451 (2011). https://doi.org/10.1007/s11596-011-0471-7

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  • DOI: https://doi.org/10.1007/s11596-011-0471-7

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